Laminin alpha 5 Antibody (CL3118) - BSA Free

Novus Biologicals | Catalog # NBP2-42391

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC, IHC-F

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3118

Format

BSA Free
View all Laminin alpha 5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from O15230, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:32157808).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin alpha 5 Antibody (CL3118) - BSA Free

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391]

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391]

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Staining in human placenta and lymph node tissues. Corresponding LAMA5 RNA-seq data are presented for the same tissues.
Western Blot: Laminin alpha 5 Antibody (CL3118) [NBP2-42391]

Western Blot: Laminin alpha 5 Antibody (CL3118) [NBP2-42391]

Western Blot: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LAMA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] -

Immunohistochemistry: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] -

Laminin-alpha-5-Antibody-CL3118-Immunohistochemistry-NBP2-42391-img0028.jpg
Western Blot: Laminin alpha 5 Antibody (CL3118) [NBP2-42391]

Western Blot: Laminin alpha 5 Antibody (CL3118) [NBP2-42391]

Western Blot: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Analysis of purified human recombinant Laminin-511, Laminin-521, Laminin-121, Laminin-221, Laminin-332 and Laminin-411.
Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391]

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391]

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Staining of human kidney shows strong membranous positivity in glomeruli, as well as in basement membrane of renal tubules.
Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391]

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391]

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Staining of human placenta shows moderate positivity in basement membrane of trophoblastic cells.
Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391]

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391]

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Staining of human colon shows moderate positivity in basement membrane of glandular cells.
Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391]

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391]

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody (CL3118) [NBP2-42391] - Staining of human lymph node shows no positivity in lymphoid cells as expected.
Laminin alpha 5 Antibody (CL3118) - BSA Free

Immunocytochemistry/ Immunofluorescence: Laminin alpha 5 Antibody (CL3118) - BSA Free [NBP2-42391] -

Altered ECM protein gene expression in liver tissue during aging. (a) Hierarchical cluster analysis of differentially expressed genes in whole liver tissue from 2‐month‐old and 22‐month‐old rats. (b) Scatter plot of gene expression of whole liver tissue from both age groups (n = 3 per age group, fold change > 2, p <.05, ANOVA). (c) Expression analyses of genes encoding for ECM proteins by qPCR. Mean expression of liver tissue samples from young rats was set to 100% (n = 8–10 for 2‐month‐old and n = 8–9 for 22‐month‐old rats, *p <.05). Immunofluorescence analysis of liver tissue sections from 2‐month‐old and 22‐month‐old rats (n = 3) with antibodies against (d, e) LAMA5, (f, g) COL1, and (h, i) COL4 (red). Cell nuclei were stained with DAPI (blue; scale bars: 20 um). (j) Proteome analysis of decellularized rat liver tissue. Mean intensities of samples from young rats were set to 100% (n = 3, *p <.05). (c, j) Data are presented as means +/- SEM Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32157808), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Laminin alpha 5 Antibody (CL3118) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin alpha 5

Components of the extracellular matrix exert myriad effects on tissues throughout the body. In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined.

Alternate Names

KIAA0533, KIAA1907, laminin alpha-5 chain, laminin subunit alpha-5, laminin, alpha 5, Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha

Gene Symbol

LAMA5

UniProt

O15230 (Human)

Additional Laminin alpha 5 Products

Product Documents for Laminin alpha 5 Antibody (CL3118) - BSA Free

Certificate of Analysis

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Product Specific Notices for Laminin alpha 5 Antibody (CL3118) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Laminin alpha 5 Antibody (CL3118) - BSA Free

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Protocols

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