Laminin alpha 5 Antibody (CL3118) - BSA Free

Novus Biologicals | Catalog # NBP3-57813

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL3118

Format

BSA Free
View all Laminin alpha 5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from O15230, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin alpha 5 Antibody (CL3118) - BSA Free

Laminin alpha 5 Antibody (CL3118) - BSA Free

Laminin alpha 5 Antibody (CL3118) - BSA Free

Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LAMA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Laminin alpha 5 Antibody (CL3118) - BSA Free

Laminin alpha 5 Antibody (CL3118) - BSA Free

Analysis in human cell line Caco-2.
Laminin alpha 5 Antibody (CL3118) - BSA Free

Laminin alpha 5 Antibody (CL3118) - BSA Free

Analysis in human placenta and lymph node tissues using NBP3-57813 antibody. Corresponding LAMA5 RNA-seq data are presented for the same tissues.
Laminin alpha 5 Antibody (CL3118) - BSA Free

Laminin alpha 5 Antibody (CL3118) - BSA Free

Analysis of purified human recombinant Laminin-511, Laminin-521, Laminin-121, Laminin-221, Laminin-332 and Laminin-411.
Laminin alpha 5 Antibody (CL3118) - BSA Free

Laminin alpha 5 Antibody (CL3118) - BSA Free

Staining of human colon shows moderate positivity in basement membrane of glandular cells.
Laminin alpha 5 Antibody (CL3118) - BSA Free

Laminin alpha 5 Antibody (CL3118) - BSA Free

Staining of human duodenum shows moderate positivity in the basement membrane of glandular cells.
Laminin alpha 5 Antibody (CL3118) - BSA Free

Laminin alpha 5 Antibody (CL3118) - BSA Free

Staining of human fallopian tube shows strong membranous positivity in smooth muscle cells of blood vessels.
Laminin alpha 5 Antibody (CL3118) - BSA Free

Laminin alpha 5 Antibody (CL3118) - BSA Free

Staining of human kidney shows strong membranous positivity in glomeruli, as well as in basement membrane of renal tubules.
Laminin alpha 5 Antibody (CL3118) - BSA Free

Laminin alpha 5 Antibody (CL3118) - BSA Free

Staining of human lymph node shows no positivity in lymphoid cells as expected.
Laminin alpha 5 Antibody (CL3118) - BSA Free

Laminin alpha 5 Antibody (CL3118) - BSA Free

Staining of human placenta shows moderate positivity in basement membrane of trophoblastic cells.

Applications for Laminin alpha 5 Antibody (CL3118) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 9 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2), 40% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin alpha 5

Components of the extracellular matrix exert myriad effects on tissues throughout the body. In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined.

Alternate Names

KIAA0533, KIAA1907, laminin alpha-5 chain, laminin subunit alpha-5, laminin, alpha 5, Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha

Gene Symbol

LAMA5

Additional Laminin alpha 5 Products

Product Documents for Laminin alpha 5 Antibody (CL3118) - BSA Free

Certificate of Analysis

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Product Specific Notices for Laminin alpha 5 Antibody (CL3118) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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