Mouse Laminin ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # KA0380

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell lysates, Cell Culture Supernatant, Plasma (EDTA, Heparin), Serum (100 uL)

Sensitivity

10 pg/mL

Assay Range

156 to 10000 pg/mL
View all Laminin ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Description

Quality control test: Standard curve. Laminin (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse laminin.

Scientific Data Images for Mouse Laminin ELISA Kit (Colorimetric)

ELISA: Mouse Laminin ELISA Kit (Colorimetric) [KA0380]

ELISA: Mouse Laminin ELISA Kit (Colorimetric) [KA0380]

ELISA: Mouse Laminin ELISA Kit (Colorimetric) [KA0380] - Standard curve for Mouse Laminin ELISA Kit (Colorimetric) [KA0380].

Kit Contents for Mouse Laminin ELISA Kit (Colorimetric)

  • 96-well plate precoated with anti- mouse Laminin antibody
  • ABC diluent buffer
  • Antibody diluent buffer
  • Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
  • Biotinylated anti-mouse Laminin antibody, dilution 1:100
  • Lyophilized mouse Laminin standard
  • Sample diluent buffer
  • TMB color developing agent
  • TMB stop solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin

Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with high molecular weight (900 kDa). Laminin is composed of a laminin alpha (400 kDa), beta (215 kDa) and gamma chain (205 kDa), whose multidomain proteins are encoded by distinct genes (LAMA, LAMB, LAMC respectively) and have several isoforms of each chain. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, reflecting diverse functions in vivo. Laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Laminins are an important and biologically active part of the basal lamina, influencing cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis, where anti-laminin antibodies can be widely used to label blood vessels and basement membranes (1). Significant quantities of laminin are found in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. Excessive serum laminin levels have been associated with fibrosis, cirrhosis and hepatitis, serious and frequent complications of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver (2). Epithelial mesenchymal transition (EMT) biomarkers include fibronectin, laminin, N-cadherin, and Slug (3).

References

1. Yang, M. Y., Chiao, M. T., Lee, H. T., Chen, C. M., Yang, Y. C., Shen, C. C., & Ma, H. I. (2015). An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior. J Biomed Mater Res B Appl Biomater, 103(3), 618-628. doi:10.1002/jbm.b.33214

2. Mak, K. M., & Mei, R. (2017). Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease. Anat Rec (Hoboken), 300(8), 1371-1390. doi:10.1002/ar.23567

3. Choi, S., Yu, J., Park, A., Dubon, M. J., Do, J., Kim, Y.,... Park, K. S. (2019). BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling. Sci Rep, 9(1), 11724. doi:10.1038/s41598-019-48190-5

Alternate Names

LAMA, Laminin A chain, laminin subunit alpha-1, laminin, alpha 1, Laminin-1 subunit alpha, Laminin-3 subunit alpha, PTBHS, S-LAM alpha, S-laminin subunit alpha

Gene Symbol

LAMA1

Additional Laminin Products

Product Documents for Mouse Laminin ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mouse Laminin ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Mouse Laminin ELISA Kit (Colorimetric)

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FAQs for Mouse Laminin ELISA Kit (Colorimetric)

Showing  1 - 4 of 4 FAQs Showing All

  • Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?

    A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.

  • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

    A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

  • Q: What is the theoretical molecular weight for your Laminin antibodies?

    A: The theoretical molecular weight for Laminin antibodies is 337 kDa.

  • Q: What research areas can this product be used in?

    A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.

  • Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?

    A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.

  • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

    A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

  • Q: What is the theoretical molecular weight for your Laminin antibodies?

    A: The theoretical molecular weight for Laminin antibodies is 337 kDa.

  • Q: What research areas can this product be used in?

    A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.

  • Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?

    A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.

  • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

    A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

  • Q: What is the theoretical molecular weight for your Laminin antibodies?

    A: The theoretical molecular weight for Laminin antibodies is 337 kDa.

  • Q: What research areas can this product be used in?

    A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.

  • Q: I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?

    A: The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.

  • Q: If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?

    A: If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.

  • Q: What is the theoretical molecular weight for your Laminin antibodies?

    A: The theoretical molecular weight for Laminin antibodies is 337 kDa.

  • Q: What research areas can this product be used in?

    A: All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.

Showing  1 - 4 of 4 FAQs Showing All
View all FAQs for ELISA Kits