Human LAT - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-40010

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.102 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)
View all LAT ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Scientific Data Images for Human LAT - Ready-To-Use ELISA Kit (Colorimetric)

Human LAT - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human LAT - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40010]

ELISA: Human LAT - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40010] - Standard Curve Reference

Kit Contents for Human LAT - Ready-To-Use ELISA Kit (Colorimetric)

  • Detection Solution A
  • Detection Solution B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: LAT

LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).

Long Name

Linker for Activation of T cells

Alternate Names

pp36

Gene Symbol

LAT

Additional LAT Products

Product Documents for Human LAT - Ready-To-Use ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human LAT - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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