Human Lefty-1 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-81150

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human Lefty-1 in samples. No significant cross-reactivity or interference between Human Lefty-1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.67%

Inter-Assay Precision (Precision between assays) CV% 5.05%

Recovery for Human Lefty-1 ELISA Kit (Colorimetric)

Recovery

87-107%

Linearity

Human Lefty-1 ELISA Kit (Colorimetric)

Scientific Data Images for Human Lefty-1 ELISA Kit (Colorimetric)

Human Lefty-1 ELISA Kit (Colorimetric)

ELISA: Human Lefty-1 ELISA Kit (Colorimetric) [NBP2-81150] -

ELISA: Human Lefty-1 ELISA Kit (Colorimetric) [NBP2-81150] - Standard Curve Reference

Kit Contents for Human Lefty-1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Lefty-1

Lefty proteins are novel TGF-beta ligands that function as antagonists of Nodal signaling. The expression of Lefty proteins on the left side of the developing mouse embryo earned this protein family its name. Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A and Lefty-B). By amino acid sequence, human Lefty-A and Lefty-B are more similar to each other (96%) than to either Lefty-1 or Lefty-2 in the mouse (81-82%). The Leftys contains some of the features of the cysteine-knot structure conserved among TGF-beta-related proteins, but also some structural differences. Specifically, they lack the alpha-helical segment important for ligand dimerization as well as the cysteine residue involved in stabilization of dimers.

Alternate Names

Leftb, Lefty-B, Lefty1

Gene Symbol

LEFTY1

Additional Lefty-1 Products

Product Documents for Human Lefty-1 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human Lefty-1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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