Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit
Novus Biologicals | Catalog # 370-0010
Key Product Details
Kit Type
Conjugate
Product Summary for Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit
Our Lightning-Link Rapid technology can be used to label small quantities of antibody with 100% recovery. The researcher simply pipettes the antibody or other biomolecule into a vial of lyophilized mixture containing the label of interest and incubates the reaction for only 15 minutes (for more details please watch the video below). As there are no desalting or dialysis steps that commonly interrupt traditional protein labeling procedures.
| Features | Benefits |
| Quick and easy to use | Save time, no special knowledge required |
| No separation steps | 100% recovery - no antibody/protein loss |
| Can be used in a wide range of applications | Flexible |
| Freeze dried | Ships at ambient temperature, long shelf-life |
| Fully scalable (10 ug to 1 g or more) | Easy transfer from R&D to manufacturing |
| Stringently QC tested | Consistent high quality, excellent batch-to-batch reproducibility |
| Large number of labels available | Experimental flexibility |
| Reliable: nearly 300 references | Successfully used in many fields of research |
The attachment of biotin to biomolecules is an important laboratory technique. Biotin binds to the tetrameric avidin proteins, including streptavidin and neutravidin, with exceptionally high affinity, and this interaction is exploited in various applications such as western blotting, immunohisthochemistry and ELISA. Our biotinylation kits are optimised to create high quality conjugates and minimise assay background.
Lightning-Link Biotin Has been optimized for two separate applications:
- Type A - Intended for assays in which a streptavidin-labeled detection reagent will be used.
- Type B - Optimised for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).
Learn more about Lightning-Link™ Conjugation Kits by reading FAQs
For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay
Product Specifications
Application Notes
Scientific Data Images for Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit
Kit Contents for Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit
- 1 or 3 glass vial(s) of Lightning-Link Rapid mix
- 1 vial of LL-Rapid Modifier reagent
- 1 vial of LL-Rapid Quencher reagent
Formulation, Preparation, and Storage
Concentration
Shipping
Storage
Product Documents for Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit
This product is manufactured by Abcam and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Citations for Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit
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FAQs for Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit
-
Q: Hi, I'm interested in buying your Lightning-Link Biotin labelling kit. I would like to couple biotin to an antibody, which would bind to an antigen in a sandwich assay format. Then streptavidin-coated nanoparticles would be used as the detection scheme. Which type is best for this purpose, type A or type B? Since Type A is for when Strep is used as a detection reagent, but Type B says its best for when Strep is immobilised to the suface of nanoparticles. In my case it is both.
A: Type B would be the preferred kit.
-
Q: What about Biotin is different for Type A and Type B 370-0010 and 371-0010?
A: The actual biotin that is used in the label is the same biotin. Biotin A has been optimized particularly for performance; it should be used when the biotinylated antibody will undergo a wash step before coming into contact with streptavidin. For example in a sandwich ELISA, free label is not a problem because a wash step will remove unbound Biotin before you add streptavidin. Biotin B has been specially developed to further minimize excess left over biotin, and should be selected if the biotinylated antibody will NOT undergo a wash step before coming into contact with streptavidin. e.g. if the biotinylated antibody will be captured on streptavidin-beads. Free, left over biotin is a problem here because it is not washed away. It is captured by the beads and so affects the signal.
-
Q: Whats the difference between Lightning-Link (R) Rapid Type A and Type B for Biotin antibody labeling?
A: Type A - Intended for assays in which a streptavidin-labeled detection reagent will be used. Type B - Optimised for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).
-
Q: Hi, I'm interested in buying your Lightning-Link Biotin labelling kit. I would like to couple biotin to an antibody, which would bind to an antigen in a sandwich assay format. Then streptavidin-coated nanoparticles would be used as the detection scheme. Which type is best for this purpose, type A or type B? Since Type A is for when Strep is used as a detection reagent, but Type B says its best for when Strep is immobilised to the suface of nanoparticles. In my case it is both.
A: Type B would be the preferred kit.
-
Q: What about Biotin is different for Type A and Type B 370-0010 and 371-0010?
A: The actual biotin that is used in the label is the same biotin. Biotin A has been optimized particularly for performance; it should be used when the biotinylated antibody will undergo a wash step before coming into contact with streptavidin. For example in a sandwich ELISA, free label is not a problem because a wash step will remove unbound Biotin before you add streptavidin. Biotin B has been specially developed to further minimize excess left over biotin, and should be selected if the biotinylated antibody will NOT undergo a wash step before coming into contact with streptavidin. e.g. if the biotinylated antibody will be captured on streptavidin-beads. Free, left over biotin is a problem here because it is not washed away. It is captured by the beads and so affects the signal.
-
Q: Whats the difference between Lightning-Link (R) Rapid Type A and Type B for Biotin antibody labeling?
A: Type A - Intended for assays in which a streptavidin-labeled detection reagent will be used. Type B - Optimised for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).
-
Q: Hi, I'm interested in buying your Lightning-Link Biotin labelling kit. I would like to couple biotin to an antibody, which would bind to an antigen in a sandwich assay format. Then streptavidin-coated nanoparticles would be used as the detection scheme. Which type is best for this purpose, type A or type B? Since Type A is for when Strep is used as a detection reagent, but Type B says its best for when Strep is immobilised to the suface of nanoparticles. In my case it is both.
A: Type B would be the preferred kit.
-
Q: What about Biotin is different for Type A and Type B 370-0010 and 371-0010?
A: The actual biotin that is used in the label is the same biotin. Biotin A has been optimized particularly for performance; it should be used when the biotinylated antibody will undergo a wash step before coming into contact with streptavidin. For example in a sandwich ELISA, free label is not a problem because a wash step will remove unbound Biotin before you add streptavidin. Biotin B has been specially developed to further minimize excess left over biotin, and should be selected if the biotinylated antibody will NOT undergo a wash step before coming into contact with streptavidin. e.g. if the biotinylated antibody will be captured on streptavidin-beads. Free, left over biotin is a problem here because it is not washed away. It is captured by the beads and so affects the signal.
-
Q: Whats the difference between Lightning-Link (R) Rapid Type A and Type B for Biotin antibody labeling?
A: Type A - Intended for assays in which a streptavidin-labeled detection reagent will be used. Type B - Optimised for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).