LIN7 Antibody

Novus Biologicals | Catalog # NBP2-15016

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all LIN7 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human LIN7. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LIN7 Antibody

Western Blot: LIN7 Antibody [NBP2-15016]

Western Blot: LIN7 Antibody [NBP2-15016]

Western Blot: LIN7 Antibody [NBP2-15016] - Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE; antibody diluted at 1:5000.
Immunohistochemistry-Paraffin: LIN7 Antibody [NBP2-15016]

Immunohistochemistry-Paraffin: LIN7 Antibody [NBP2-15016]

Immunohistochemistry-Paraffin: LIN7 Antibody [NBP2-15016] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.
LIN7 Antibody

Immunocytochemistry/ Immunofluorescence: LIN7 Antibody [NBP2-15016] -

Immunocytochemistry/ Immunofluorescence: LIN7 Antibody [NBP2-15016] - LIN7 antibody [N1C3] detects LIN7 protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: LIN7 protein stained by LIN7 antibody [N1C3] (NBP2-15016) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
LIN7 Antibody

Western Blot: LIN7 Antibody [NBP2-15016] -

Western Blot: LIN7 Antibody [NBP2-15016] - Sample (30 ug of whole cell lysate)
A: zebrafish eye
12% SDS PAGE
NBP2-15016 diluted at 1:1000
LIN7 Antibody

Immunohistochemistry: LIN7 Antibody [NBP2-15016] -

Immunohistochemistry: LIN7 Antibody [NBP2-15016] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using LIN7 antibody [N1C3] (NBP2-15016) at 1:300 dilution.
LIN7 Antibody

Immunohistochemistry: LIN7 Antibody [NBP2-15016] -

Immunohistochemistry: LIN7 Antibody [NBP2-15016] - LIN7 antibody [N1C3] detects Lin7a protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
LIN7 antibody [N1C3] (NBP2-15016) dilution: 1:200.

Applications for LIN7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LIN7

Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells.

Alternate Names

hLin-7, LIN7, lin-7 homolog A (C. elegans), Lin-7A, MALS1, MALS-1MGC148143, Mammalian lin-seven protein 1, Tax interaction protein 33, TIP-33mammalian LIN-7 1, veli-1, VELI1protein lin-7 homolog A, vertebrate LIN7 homolog 1, Vertebrate lin-7 homolog 1

Gene Symbol

LIN7A

UniProt

O14910 (Human)

Additional LIN7 Products

Product Documents for LIN7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for LIN7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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