Recombinant Human LPCAT1 His Protein

Name: Recombinant Human LPCAT1 His Protein
Brand: Novus Biologicals
SKU: NBP2-23206
Price: 329 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-23206

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE

View all LPCAT1 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 79-534 of Human LPCAT1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSSAEKEPE QPPALWRKVV DFLLKAIMRT MWFAGGFHRV AVKGRQALPT EAAILTLAPH SSYFDAIPVT MTMSSIVMKA ESRDIPIWGT LIQYIRPVFV SRSDQDSRRK TVEEIKRRAQ SNGKWPQIMI FPEGTCTNRT CLITFKPGAF IPGAPVQPVV LRYPNKLDTI TWTWQGPGAL EILWLTLCQF HNQVEIEFLP VYSPSEEEKR NPALYASNVR RVMAEALGVS VTDYTFEDCQ LALAEGQLRL PADTCLLEFA RLVRGLGLKP EKLEKDLDRY SERARMKGGE KIGIAEFAAS LEVPVSDLLE DMFSLFDESG SGEVDLRECV VALSVVCRPA RTLDTIQLAF KMYGAQEDGS VGEGDLSCIL KTALGVAELT VTDLFRAIDQ EEKGKITFAD FHRFAEMYPA FAEEYLYPDQ THFESCAETS PAPIPNGFCA DFSPENSDAG RKPVRKKLD

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

53.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human LPCAT1 His Protein

SDS-PAGE: Recombinant Human LPCAT1 His Protein [NBP2-23206]

SDS-Page: LPCAT1 Protein [NBP2-23206]

Formulation, Preparation, and Storage

NBP2-23206

Formulation	20 mM Tris-HCl buffer (pH 8.0), 1 M UREA, 10% glycerol
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LPCAT1

Lysophosphatidylcholine acyltransferase 1, also known as LPCAT1, belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. It is a key enzyme for remodeling phospholipids, including phosphatidylcholine. LPCAT1 possesses both acyltransferase and acetyltransferase activities. It mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Recombinant human LPCAT1 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

1-acylglycerophosphocholine O-acyltransferase, Acetyl-CoA:lyso-PAF acetyltransferase, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, acyl-CoA:lysophosphatidylcholine acyltransferase 1, acyltransferase like 2, Acyltransferase-like 2, AYTL2, EC 2.3.1.-, EC 2.3.1.23,1-alkylglycerophosphocholine O-acetyltransferase, EC 2.3.1.67, FLJ12443, FLJ41609, LPC acyltransferase 1, lpcat, LPCAT-1, Lyso-PAF acetyltransferase, LysoPAFAT, LysoPC acyltransferase 1, lysophosphatidylcholine acyltransferase 1, PFAAP3, Phosphonoformate immuno-associated protein 3, regulated by phosphonoformate

Gene Symbol

LPCAT1

Additional LPCAT1 Products

Product Documents for Recombinant Human LPCAT1 His Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human LPCAT1 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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