LRH-1/NR5A2 Antibody (JE30-50)

Novus Biologicals | Catalog # NBP3-32660

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE30-50 expressed in HEK293
View all LRH-1/NR5A2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human LRH-1/NR5A2 aa 442-541 / 541. (Uniprot: O00482)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LRH-1/NR5A2 Antibody (JE30-50)

LRH-1/NR5A2 Antibody (JE30-50)

Western Blot: LRH-1/NR5A2 Antibody (JE30-50) [NBP3-32660]

Western Blot: LRH-1/NR5A2 Antibody (JE30-50) [NBP3-32660] - Western blot analysis of LRH-1/NR5A2 on different lysates with Rabbit anti-LRH-1/NR5A2 antibody (NBP3-32660) at 1/1,000 dilution.

Lane 1: MCF7 cell lysate (15 ug/Lane)
Lane 2: 293T cell lysate (15 ug/Lane)
Lane 3: HepG2 cell lysate (15 ug/Lane)
Lane 4: A549 cell lysate (15 ug/Lane)
Lane 5: PANC-1 cell lysate (15 ug/Lane)
Lane 6: SW1990 cell lysate (15 ug/Lane)
Lane 7: Mouse liver tissue lysate (30 ug/Lane)
Lane 8: Rat liver tissue lysate (30 ug/Lane)
Lane 9: Mouse brain tissue lysate (30 ug/Lane)
Lane 10: Rat brain tissue lysate (30 ug/Lane)
Lane 11: Mouse pancreas tissue lysate (30 ug/Lane)

Predicted band size: 61 kDa
Observed band size: 65 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32660) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for LRH-1/NR5A2 Antibody (JE30-50)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LRH-1/NR5A2

Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells.

Long Name

Liver Receptor Homolog 1/Nuclear Receptor Subfamily 5, Group A, Member 2

Alternate Names

B1F2, FTZ-F1 beta, LRH1, NR5A2

Gene Symbol

NR5A2

Additional LRH-1/NR5A2 Products

Product Documents for LRH-1/NR5A2 Antibody (JE30-50)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LRH-1/NR5A2 Antibody (JE30-50)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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