LRP-4 Antibody (HL2080) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25552
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2080 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragment of mouse LRP-4
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
245 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for LRP-4 Antibody (HL2080) - Azide and BSA Free
Western Blot: LRP-4 Antibody (HL2080) - Azide and BSA Free [NBP3-25552] -
Western Blot: LRP-4 Antibody (HL2080) - Azide and BSA Free [NBP3-25552] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with LRP4 antibody [HL2080] (NBP3-25552) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Western Blot: LRP-4 Antibody (HL2080) - Azide and BSA Free [NBP3-25552] -
Western Blot: LRP-4 Antibody (HL2080) - Azide and BSA Free [NBP3-25552] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with LRP4 antibody [HL2080] (NBP3-25552) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Western Blot: LRP-4 Antibody (HL2080) - Azide and BSA Free [NBP3-25552] -
Western Blot: LRP-4 Antibody (HL2080) - Azide and BSA Free [NBP3-25552] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with LRP4 antibody [HL2080] (NBP3-25552) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/Immunofluorescence: LRP-4 Antibody (HL2080) - Azide and BSA Free [NBP3-25552] -
Immunocytochemistry/Immunofluorescence: LRP-4 Antibody (HL2080) - Azide and BSA Free [NBP3-25552] - LRP4 antibody [HL2080] detects LRP4 protein at cell membrane and nucleus by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LRP4 stained by LRP4 antibody [HL2080] (NBP3-25552) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody diluted at 1:500. Blue: Fluoroshield with DAPI.Western Blot: LRP-4 Antibody (HL2080) - Azide and BSA Free [NBP3-25552] -
Western Blot: LRP-4 Antibody (HL2080) - Azide and BSA Free [NBP3-25552] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with LRP4 antibody [HL2080] (NBP3-25552) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for LRP-4 Antibody (HL2080) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LRP-4
Long Name
LDL Receptor-related Protein 4
Alternate Names
LRP10, LRP4
Gene Symbol
LRP4
Additional LRP-4 Products
Product Documents for LRP-4 Antibody (HL2080) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for LRP-4 Antibody (HL2080) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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