LRRC32/GARP Antibody (GARP5) - BSA Free

Novus Biologicals | Catalog # NBP2-37710

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GARP5

Format

BSA Free
View all LRRC32/GARP Primary Antibodies »

Product Specifications

Immunogen

Purified human sGARP protein.

Specificity

Antibody recognizes GARP / LRRC32, an approximately 80 kDa glycoprotein expressed e.g. on the surface of megakaryocytes, platelets and activated Treg cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LRRC32/GARP Antibody (GARP5) - BSA Free

LRRC32/GARP Antibody (GARP5) - BSA Free

Western Blot: Mouse Monoclonal LRRC32/GARP Antibody (GARP5) [NBP2-37710} -

Western Blot: Mouse Monoclonal LRRC32/GARP Antibody (GARP5) [NBP2-37710} - Western blotting analysis was performed on RIPA buffer extracts of thrombocytes, leukocytes, and Jurkat cells mixed with hot reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody GARP5 (2 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 ug/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.GARP was detected at ~80 kDa in thrombocytes.

Applications for LRRC32/GARP Antibody (GARP5) - BSA Free

Application
Recommended Usage

Western Blot

1-2 ug/ml
Application Notes
Western Blot: reducing conditions

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: LRRC32/GARP

LRRC32 encodes a type I membrane protein which contains 20 leucine-rich repeats. Alterations in the chromosomal region 11q13-11q14 are involved in several pathologies. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same isoform.

Long Name

Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant

Alternate Names

D11S833E, GARP, Garpin

Gene Symbol

LRRC32

Additional LRRC32/GARP Products

Product Documents for LRRC32/GARP Antibody (GARP5) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for LRRC32/GARP Antibody (GARP5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Regulatory T Cells (Tregs)

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