LRRC4 (Leu-rich repeat/LRR-containing glycoprotein 4), LRRC4B and LRRC4C, also called NGL-2 (netrin-G ligand-2), NGL-3 and NGL-1, respectively, are post-synaptic adhesion molecules of the LRR protein family that induce excitatory synapse formation. Mature LRRC4 family members are type I transmembrane proteins with an extracellular region that contains nine LRRs, a C2-type Ig-like domain, and a Thr-rich segment. LRRC4 family members share ~55% aa identity, but each recognizes different ligands. LRRC4 and LRRC4C recognize Netrin-G2 and Netrin-G3, respectively, while LRRC4B recognizes the receptor tyrosine phosphatases LAR/PTPRF, PTPR delta and PTPR sigma.
LRRC4 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-05099
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 544-653 of human LRRC4 (NP_071426.1). LIVFYKLRKRHQQRSTVTAARTVEIIQVDEDIPAATSAAATAAPSGVSGEGAVVLPTIHDHINYNTYKPAHGAHWTENSLGNSLHPTVTTISEPYIIQTHTKDKVQETQI
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for LRRC4 Antibody - BSA Free
Western Blot: LRRC4 AntibodyBSA Free [NBP3-05099]
Western Blot: LRRC4 Antibody [NBP3-05099] - Analysis of extracts of various cell lines, using LRRC4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL BasicApplications for LRRC4 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:1000-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: LRRC4
Long Name
Leucine-rich Repeat Containing 4
Alternate Names
BAG, NAG14
Gene Symbol
LRRC4
Additional LRRC4 Products
Product Documents for LRRC4 Antibody - BSA Free
Certificate of Analysis
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Product Specific Notices for LRRC4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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