LRRC55 Antibody - BSA Free

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• LRRC55 Antibody

  Name: Ping Dong

  Application: Immunohistochemistry-Frozen

  Sample Tested: Adult mouse brain tissue

  Species: Mouse

  Verified Customer | Posted 06/17/2021

  Lrrc55 is known highly expressed in the medial habenula (MHb) of mouse brain. However, the NBP1-81890-25ul Rabbit Polyclonal LRRC55 Antibody did not have any signal in the MHb.

  1. Wash in PBS 10min x 3 2. Incubation in 5% goat serum and 0.3% Triton X-100 for 2h at RT 3. 5% goat serum and 0.3% Triton X-100 4. Rabbit Polyclonal LRRC55 Antibody (1:50) 5. Goat anti-rabbit 488 6. PBS 10min x 3 7. DAPI 5min at RT 8. PBS 10min x 3 Mice were transcardially perfused with phosphate-buffered saline (PBS) followed by 4% paraformaldehyde. The brain was removed and post-fixed in 4% paraformaldehyde overnight at 4°C and dehydrated in 30% sucrose for 48 h. Coronal section (50 μm) containing the medial habenula were collected using a cryostat. The sections were rinsed 3 times with PBS for 10 min each and blocked with 5% goat serum and 0.3% Triton X-100 for 2 hours at room temperature and incubated for overnight at 4°C with following primary antibodies: anti-Lrrc55 (1:50, NBP1-81890-25UL). After 3 rinses with PBS for 10 min, secondary antibodies (1:1000, conjugated with Alexa 594) were incubated for 2 hours at room temperature. Then the sections were washed 3 times with PBS for 10 min each and stained with DAPI (1:10000 of 5 mg/mL). Images were acquired using a Zeiss 780 inverted confocal microscope.

  
  Bio-Techne Response

  This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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