Human Lung Whole Tissue Lysate (Adult Whole Normal)

Novus Biologicals | Catalog # NB820-59237

Novus Biologicals
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Key Product Details

Species

Human

Applications

Immunoprecipitation, Western Blot

Product Specifications

Application Notes

Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Specificity

Total Protein - Human Adult Normal Tissue: Lung: Left Upper Lobe

Type

Tissue

Life Stage

Adult

Tissue

Respiratory

Protein State

Native

Tissue Condition

Normal

Subcellular Fraction

Whole

Formulation, Preparation, and Storage

Formulation

HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors

Concentration

5.0 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Lung

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Additional Lung Products

Product Documents for Human Lung Whole Tissue Lysate (Adult Whole Normal)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Lung Whole Tissue Lysate (Adult Whole Normal)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Citations for Human Lung Whole Tissue Lysate (Adult Whole Normal)

Customer Reviews for Human Lung Whole Tissue Lysate (Adult Whole Normal) (1)

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  • Human Lung Whole Tissue Lysate (Adult Whole Normal)
    Name: Ming Lu
    Verified Customer | Posted 05/15/2024
    Human Lung Whole Tissue Lysate (Adult Whole Normal) 30ug/lane was loaded to SDS-PAGE gel and transferred to membrane, Ponceau S staining shows the proteins have been degraded, and skin lysates can be used as a non-degraded control.
    Human Lung Whole Tissue Lysate (Adult Whole Normal) NB820-59237
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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FAQs for Human Lung Whole Tissue Lysate (Adult Whole Normal)

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    • Q: For NB820-59237 lysate, I'd like to know the extraction procedure. Which buffer has been used? Protease, Phosphotase Inhibitors? And how will the lysate be delivered (which buffer and concentration)?
      A: A total protein extraction kit was used for the extraction of the total protein lysate. This kit uses a TM Buffer (HEPES (pH7.9), MgCl2, Kcl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, Sodium OrthoVanadate) and a cocktail of protease inhibitors. The protein is delivered at 5 ug/ul concentration. We have protease inhibitor cocktail in the lysate, but we don't release the specific protease inhibitor information.
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