Human Lung Whole Tissue Lysate (Adult Whole Normal)

Novus Biologicals | Catalog # NB820-59240

Novus Biologicals
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Key Product Details

Species

Human

Applications

Western Blot

Product Summary for Human Lung Whole Tissue Lysate (Adult Whole Normal)

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
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Product Specifications

Application Notes

Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Specificity

Total Protein - Human Adult Normal Tissue: Lung: Right Upper Lobe

Type

Tissue

Life Stage

Adult

Tissue

Respiratory

Protein State

Native

Tissue Condition

Normal

Subcellular Fraction

Whole

Formulation, Preparation, and Storage

Formulation

HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors

Concentration

5.0 mg/ml

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: Lung

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Additional Lung Products

Product Documents for Human Lung Whole Tissue Lysate (Adult Whole Normal)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Lung Whole Tissue Lysate (Adult Whole Normal)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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FAQs for Human Lung Whole Tissue Lysate (Adult Whole Normal)

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  • Q: For NB820-59240 lysate, I'd like to know the extraction procedure. Which buffer has been used? Protease, Phosphotase Inhibitors? And how will the lysate be delivered (which buffer and concentration)?

    A: A total protein extraction kit was used for the extraction of the total protein lysate. This kit uses a TM Buffer (HEPES (pH7.9), MgCl2, Kcl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, Sodium OrthoVanadate) and a cocktail of protease inhibitors. The protein is delivered at 5 ug/ul concentration. We have protease inhibitor cocktail in the lysate, but we don't release the specific protease inhibitor information.

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