M1 Macrophage Flow Cytometry Panel
R&D Systems | Catalog # FMC-P-006
Key Product Details
Features
Species
Conjugate
Scientific Data Images for M1 Macrophage Flow Cytometry Panel
Multicolor flow cytometry panel to identify M1 Macrophages.
Monocytes were isolated from PBMCs via adherence depletion for 5 hours. Non-adherent cells were removed. Adherent cells were incubated for 6 days in C3 +10% huAB media with 50 ng/ml Recombinant Human GM-CSF (215-GM), media and cytokines are refreshed on day 3. For M1 polarization, 24 hours prior to staining, cells are incubated with 50 ng/ml human GM-CSF (215-GM), 50 ng/ml Recombinant Human IFN-gamma (285-IF), and 50 ng/ml LPS. Cells were stained with Anti-human CD3 Alexa Fluor ® 405, CD20 Alexa Fluor 405, CD56 Alexa Fluor 700, CD86 FITC, HLA-DR PerCP, CD11c PE,and CD38 APC. Cells were previously gated on Live CD3-CD20-CD56-. Find Staining Protocol Here.
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Background: Flow Cytometry Panel
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Product Specific Notices for M1 Macrophage Flow Cytometry Panel
For research use only
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