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Recombinant Human IFN-gamma Protein

R&D Systems | Catalog # 285-IF

GMP Version Available: 285-GMP
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Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human IFN-gamma Protein (285-IF)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

CAA31639

Applications

Bioactivity
View all IFN-gamma Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IFN-gamma protein
Gln24-Gln166 with an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

16.9 kDa

SDS-PAGE

17 kDa, reducing conditions

Activity

Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129.
The ED50 for this effect is 0.15-0.75 ng/mL.

Reviewed Applications

Read 26 reviews rated 4.7 using 285-IF in the following applications:

Scientific Data Images for Recombinant Human IFN-gamma Protein

Recombinant Human IFN‑ gamma Protein SEC-MALS.

Recombinant Human IFN-gamma (Catalog # 285-IF) has a molecular weight (MW) of 34.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human IFN-gamma Protein SDS-PAGE

Recombinant Human IFN-gamma Protein SDS-PAGE

1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.
Recombinant Human IFN-gamma Protein Bioactivity

Recombinant Human IFN-gamma Protein Bioactivity

Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 285-IF
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with BSA as a carrier protein.
Reconstitution Reconstitute at 200-500 μg/mL in sterile, deionized water.  
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 285-IF/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80.
Reconstitution Reconstitute at 0.2 mg/mL in sterile, deionized water. 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59%‑64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37%‑43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta  subunits) to form the functional receptor complex of two alpha and two beta  subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

References

  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Gene Symbol

IFNG

UniProt

CAA31639

Additional IFN-gamma Products

Product Documents for Recombinant Human IFN-gamma Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 285-IF (with carrier)
Download SDS 285-IF/CF (carrier free)

Product Specific Notices for Recombinant Human IFN-gamma Protein

For research use only

Citations for Recombinant Human IFN-gamma Protein

Customer Reviews for Recombinant Human IFN-gamma Protein (26)

4.7 out of 5
26 Customer Ratings
5 Stars
73%
4 Stars
19%
3 Stars
8%
2 Stars
0%
1 Stars
0%

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Showing  1 - 5 of 26 reviews Showing All
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  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: Cell migration/motility
    Verified Customer | Posted 10/09/2025
    IFN-induced TNF-alpha
    Recombinant Human IFN-gamma Protein 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Lana Jagannathan
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 08/26/2024
    Recombinant Human IFN-gamma Protein 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: Flow Cytometry
    Verified Customer | Posted 06/24/2022
    Black -> HLA-E surface expression in T cells Grey -> HLA-E surface expression in T cells upon IFNg stimulation (12h)
    Recombinant Human IFN-gamma Protein 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/24/2022
    More than a hundred-fold increase for a given target gene in immune cells stimulated with IFNg for 6h compared to controls (for basal level).
    Recombinant Human IFN-gamma Protein 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Verified Customer | Posted 06/24/2022
    Lane 1: PBMCs, unstimulated Lane 2: PBMCs, stimulated with IFNg for 6h Lane 3: PBMCs, stimulated with IFNg for 24h Target: HLA detection
    Recombinant Human IFN-gamma Protein 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 05/21/2021
  • Recombinant Human IFN-gamma Protein
    Name: Marija Pieper
    Application: Apoptosis assay
    Verified Customer | Posted 03/11/2021
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Verified Customer | Posted 12/18/2020
    Recombinant Human IFN-gamma Protein 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 11/20/2020
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/16/2020
    Used the to stimulate ex vivo skin culture
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/09/2020
    HCT-116 cells were treated with hr-IFN-gamma for 24 hours and intracellular IDO-1 expression was measured.
    Recombinant Human IFN-gamma Protein 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/08/2020
    Recombinant Human IFN-gamma Protein 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/06/2020
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 07/26/2019
  • Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 07/25/2019
    CD38 transcript levels measured by qPCR after 24 hours treatment with 10 ng/mL IFN-g in MV-4-11 cells
    Recombinant Human IFN-gamma Protein 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/09/2019
    Recombinant Human IFN-gamma Protein 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/09/2019
    The expression of HLA-DR increased with MDAMB231 cells incubated with 1000 IU mL–1 IFN-gamma for 72 h
    Recombinant Human IFN-gamma Protein 285-IF
  • Name: Mehdi Alavi
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 03/19/2019
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 11/16/2018
    Recombinant Human IFN-gamma Protein 285-IF
  • Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/08/2018
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 10/07/2018
    HT-29 Cell lines were treated with 100 U/ml IFN gamma, after which HLA-DR expression was examined by flow cytometer
    Recombinant Human IFN-gamma Protein 285-IF
  • Name: Pia Muyot
    Verified Customer | Posted 04/26/2017
  • Recombinant Human IFN-gamma Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 07/21/2016
    IFN-g was used to stimulate whole blood. 100ng/mL final concentration was used in 1mL whole blood for 15 minutes at 37C. The cells were fixed and stored in -80C until further analysis on CyTOF
  • Recombinant Human IFN-gamma Protein
    Name: Adam Guess
    Application: In vivo study
    Verified Customer | Posted 07/13/2016
    IFN-gamma treatment (solid line) induces HLA-DR expression in cultured MSC as compared to control cells (filled histogram).
    Recombinant Human IFN-gamma Protein 285-IF
  • Recombinant Human IFN-gamma Protein
    Name: shu wang
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 05/31/2016
  • Recombinant Human IFN-gamma Protein
    Name: SOjan Abraham
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/21/2016

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FAQs for Recombinant Human IFN-gamma Protein

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  • Q: Why does Recombinant Human IFN-gamma protein, Catalog # 285-IF, have the amio acid substitution R160Q?

    A: The sequence of R&D Systems' rhIFN-gamma, Catalog # 285-IF,  is based on the the following publication in Nature:[https://www.ncbi.nlm.nih.gov/pubmed/?term=Gray+1982+503] Note that in figure 3, the article describes a Gln (Q) in position 160.

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