Human MAG/Siglec-4a ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-81156

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL
View all MAG/Siglec-4a ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human MAG/Siglec-4a in samples. No significant cross-reactivity or interference between Human MAG/Siglec-4a and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.15%

Inter-Assay Precision (Precision between assays) CV% 4.76%

Recovery for Human MAG/Siglec-4a ELISA Kit (Colorimetric)

Recovery

86-105%

Linearity

Human MAG/Siglec-4a ELISA Kit (Colorimetric)

Scientific Data Images for Human MAG/Siglec-4a ELISA Kit (Colorimetric)

Human MAG/Siglec-4a ELISA Kit (Colorimetric)

ELISA: Human MAG/Siglec-4a ELISA Kit (Colorimetric) [NBP2-81156] -

ELISA: Human MAG/Siglec-4a ELISA Kit (Colorimetric) [NBP2-81156] - Standard Curve Reference

Kit Contents for Human MAG/Siglec-4a ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MAG/Siglec-4a

Myelin-associated glycoprotein (MAG), a membrane glycoprotein of 100 kDa, is thought to be involved in the process of myelination (1). Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and MAG (2). MAG has been implicated in inhibition of nerve regeneration in the CNS. This results from interactions between MAG and the Nogo receptor and gangliosides on the apposing axon, which generates intracellular inhibitory signals in the neuron (3). Results also suggest that MAG binds to a specific receptor and initiates a signal transduction cascade to effect inhibition. These results indicate that soluble dMAG detected in vivo could contribute to the lack of regeneration in the mammalian CNS after injury (4).

Long Name

Myelin-associated Glycoprotein

Alternate Names

Siglec-4a, Siglec4a

Gene Symbol

MAG

Additional MAG/Siglec-4a Products

Product Documents for Human MAG/Siglec-4a ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human MAG/Siglec-4a ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Glial Lineage Markers

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