MAGEA3 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-15499

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all MAGEA3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MAGEA3 (NP_005353.1). GLLIIVLAIIAREGDCAPEEKIWEELSVLEVFEGREDSILGDPKKLLTQHFVQENYLEYRQVPGSDPACYEFLWGPRALVETSYVKVLHHMVKISGGPHIS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit MAGEA3 Antibody - Azide and BSA Free (NBP3-15499) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MAGEA3 Antibody - Azide and BSA Free

Western Blot: MAGEA3 AntibodyAzide and BSA Free [NBP3-15499]

Western Blot: MAGEA3 AntibodyAzide and BSA Free [NBP3-15499]

Western Blot: MAGEA3 Antibody [NBP3-15499] - Western blot analysis of extracts of various cell lines, using MAGEA3 Rabbit pAb (NBP3-15499) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Applications for MAGEA3 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MAGEA3

MAGEA3, also known as Melanoma-associated antigen 3, is a 34.7 kDa, 314 amino acid protein that is involved in increasing ubiquitin ligase activity as a member of the MAGEA family. Current research is being conducted to study the effects of the protein on diseases and disorders such as bile duct cancer, dyskeratosis, ocular melanoma, germ cell tumors, multiple types of carcinomas, avian influenza, testicular cancer, lung cancer, and lymphoma. The protein interacts with other proteins such as TRIM28, UBC, HTT, and SUMO2.

Long Name

Melanoma-Associated Antigen 3

Alternate Names

CT1.3, MAGE3

Gene Symbol

MAGEA3

Additional MAGEA3 Products

Product Documents for MAGEA3 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MAGEA3 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Cancer Stem Cell Markers

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