MAML3 Antibody
Novus Biologicals | Catalog # NB100-2129
![Western Blot: MAML3 Antibody [NB100-2129]](https://resources.rndsystems.com/images/products/MAML3-Antibody-Western-Blot-NB100-2129-img0005.jpg "Western Blot: MAML3 Antibody [NB100-2129]")
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Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1100 and the C-terminus (residue 1133) human Mastermind-Like 3 using the numbering given in entry NP_061187.2 (GeneID 55534).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MAML3 Antibody
Western Blot: MAML3 Antibody [NB100-2129]
Western Blot: MAML3 Antibody [NB100-2129] - Detection of Human and Mouse MAML3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAML3 antibody NB100-2129 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2129]](https://resources.rndsystems.com/images/products/MAML3-Antibody-Immunohistochemistry-NB100-2129-img0006.jpg "Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2129]")
Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2129]
Immunohistochemistry-Paraffin: MAML3 Antibody [NB100-2129] - Detection of human MAML3 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAML3 (NB100-2129). Detection: DAB![Immunoprecipitation: MAML3 Antibody [NB100-2129]](https://resources.rndsystems.com/images/products/MAML3-Antibody-Immunoprecipitation-NB100-2129-img0007.jpg "Immunoprecipitation: MAML3 Antibody [NB100-2129]")
Immunoprecipitation: MAML3 Antibody [NB100-2129]
Immunoprecipitation: MAML3 Antibody [NB100-2129] - Detection of human MAML3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAML3 antibody NB100-2129 (lot NB100-2129-2) used for IP at 6 ug per reaction. MAML3 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2129-1) and rabbit anti-MAML3 antibody NB100-2128 For blotting immunoprecipitated MAML3, NB100-2129 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for MAML3 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:200- 1:1000
Immunoprecipitation
1-4 ug/mg of lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval withTris-EDTA pH9.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MAML3
Alternate Names
CAG/CTG 3, ERDA3, MAM-2, mam-3, mastermind-like 3 (Drosophila), mastermind-like protein 3, polyglutamine rich, trinucleotide repeat containing 3
Gene Symbol
MAML3
UniProt
Additional MAML3 Products
Product Documents for MAML3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MAML3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for MAML3 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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