Human MANF ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-81157

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 uL)

Sensitivity

9.38 pg/mL

Assay Range

15.63 - 1000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human MANF in samples. No significant cross-reactivity or interference between Human MANF and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV 4.46

Inter-Assay Precision (Precision between assays) %CV 5.66

Recovery for Human MANF ELISA Kit (Colorimetric)

Recovery

85 - 112%

Linearity

Human MANF ELISA Kit (Colorimetric)

Scientific Data Images for Human MANF ELISA Kit (Colorimetric)

Human MANF ELISA Kit (Colorimetric)

ELISA: Human MANF ELISA Kit (Colorimetric) [NBP2-81157] -

ELISA: Human MANF ELISA Kit (Colorimetric) [NBP2-81157] - Standard Curve Reference

Kit Contents for Human MANF ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MANF

MANF is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and promotes cell proliferation. The protein was initially thought to be longer at the N-terminus and to contain an arginine-rich region but transcribed evidence indicates a smaller open reading frame that does not encode the arginine tract. The presence of a specific mutation changing the previously numbered codon 50 from ATG to AGG, or deletion of that codon, has been reported in a variety of solid tumors. With the protein size correction, this codon is now identified as the initiation codon. [provided by RefSeq]

Long Name

Mesencephalic Astrocyte-derived Neurotrphic Factor

Alternate Names

ARMET, ARP

Gene Symbol

MANF

Additional MANF Products

Product Documents for Human MANF ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human MANF ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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