Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Mas Primary Antibodies »

Product Specifications

Immunogen

KLH conjugated synthetic peptide made to the C-terminus of human MAS1. [UniProt# P04201]

Localization

Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Mas Antibody - BSA Free (NLS1531) is a polyclonal antibody validated for use in IHC, Flow and ICC/IF. Anti-Mas Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Mas Antibody - BSA Free

Immunohistochemistry: Mas Antibody - BSA Free [NLS1531]

Immunohistochemistry: Mas Antibody - BSA Free [NLS1531]

Immunohistochemistry: Mas Antibody [NLS1531] - Analysis of MAS1 in mouse kidney using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: Mas Antibody - BSA Free [NLS1531]

Immunohistochemistry-Paraffin: Mas Antibody - BSA Free [NLS1531]

Mas-Antibody-Immunohistochemistry-Paraffin-NLS1531-img0003.jpg
Immunohistochemistry-Paraffin: Mas Antibody - BSA Free [NLS1531]

Immunohistochemistry-Paraffin: Mas Antibody - BSA Free [NLS1531]

Immunohistochemistry-Paraffin: Mas Antibody [NLS1531] - Analaysis of Brain, Neurons and Glia using NLS1531 at 15 ug/ ml.
Mas Antibody - BSA Free

Immunohistochemistry: Mas Antibody - BSA Free [NLS1531] -

(a) Representative immunolocalisation images of AT1R, AT2R, MasR, and ACE2 in adult WKY rats and aged WKY rats. Mean data for aortic expression of the (b) AT1R, (c) AT2R, (d) MasR, and (e) ACE2 expressed as relative fluorescent units in adult (n = 5) and aged (n = 4) WKY rats. ***P < 0.001 versus adult WKY rats.
Mas Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Mas Antibody - BSA Free [NLS1531] -

Immunocytochemistry/ Immunofluorescence: Mas Antibody - BSA Free [NLS1531] - (a) Representative immunolocalisation images of AT1R, AT2R, MasR, & ACE2 in adult WKY rats & aged WKY rats. Mean data for aortic expression of the (b) AT1R, (c) AT2R, (d) MasR, & (e) ACE2 expressed as relative fluorescent units in adult (n = 5) & aged (n = 4) WKY rats. ***P < 0.001 versus adult WKY rats. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22187625), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Mas Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Mas Antibody - BSA Free [NLS1531] -

Immunocytochemistry/ Immunofluorescence: Mas Antibody - BSA Free [NLS1531] - Immunofluorescence studies in HEK293T cells overexpressing c-myc tagged MasR using MasR antibodies.Images of fluorescence signal corresponding to secondary cyanine (Cy3)-conjugated antibody (A), nuclear Hoechst 33258 staining (B), merged images (C) & bright field (D) are shown. In cells transfected with the pcDNA 3.1/c-myc-MasR construct, antibodies NLS-1531 & sc-54682 generated a similar staining pattern to that generated with the anti c-myc antibody. No staining was observed with any of these antibodies in cells transfected with the empty vector pcDNA 3.1. Antibody sc-135063 was capable of staining the plasma membrane of cells overexpressing the MasR but also generated widespread signals in cells transfected with the empty vector. For antibody AAR-013 there was no staining associated with the cell membrane. Intense immunocytochemical staining of identical distribution & intensity was revealed in cells transfected with the empty vector & those transfected with the c-myc-MasR construct. Images are representative of 3 independent experiments. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0183278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Mas Antibody - BSA Free

Immunohistochemistry: Mas Antibody - BSA Free [NLS1531] -

Immunohistochemistry: Mas Antibody - BSA Free [NLS1531] - Immunohistochemistry in heart & kidney from wild-type (WT) & MasR-KO mice.Negative controls performed by omitting the primary antibody demonstrated minimal background immunostaining in heart (A) & kidney (B) sections. (A) In heart sections, NLS-1531 antibody stained predominantly the cytoplasm of cardiomyocytes with similar intensity in WT & MasR-KO hearts. The AAR-013 antibody stained the cardiomyocytes nucleus & weaker staining was observed in their cytoplasm. The same pattern was found in heart sections of MasR-KO mice. (B) In kidney sections, for NLS-1531 antibody, staining was mostly restricted to cytoplasm of numerous tubules cells with similar intensity in WT & MasR-KO kidneys. Antibody AAR-013 stained most intensely tubules cells nucleus & weaker staining was observed in their cytoplasm. Glomeruli were also stained positively. The same pattern was found in kidney sections of MasR-KO. Preincubation of the AAR-013 antibody with the blocking peptide provided by the vendors eliminated the immunohistochemical nuclear staining both in WT & MasR-KO mice kidney sections. Images are representative of n = 3. Bar, 50 μm. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0183278), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Mas Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 26386115)

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported in scientific literature (PMID 22187625)

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Frozen

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500. Use reported in scientific literature (PMID 22187625)
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reviewed Applications

Read 1 review rated 5 using NLS1531 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mas

MAS1 oncogene (Proto-oncogene Mas, MAS1) is a GTP binding protein coupled receptor which shares a conserved structural motif with other proteins of its class. When activated, MAS1 is a modulator of a critical step in the growth-regulating pathway to trigger oncogenes (PMID: 20674894). Evidence has shown that the MAS1 oncogene acts as a receptor for angiotensin (Ang)-(1-7), however it is not fully known how Intracellular signaling through Mas1 occurs (PMID: 19461648). Furthermore, research proves that in tandom with Ang-(1-7), the MAS1 receptor is expressed in the ovarian stroma of post menopausal women and in male testis (PMID: 20674894, 20361351). Additionally, together with Ang- (1-7) MAS1 may be an important component of normal spermatogenesis as low expression levels were found in infertile men with impaired spermatogenesis (PMID: 20361351).

Long Name

MAS1 Oncogene

Alternate Names

MAS1

Entrez Gene IDs

4142 (Human)

Gene Symbol

MAS1

UniProt

P04201 (Human)

Additional Mas Products

Product Documents for Mas Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Mas Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Mas Antibody - BSA Free

Customer Reviews for Mas Antibody - BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested:
    Species: Human
    Verified Customer | Posted 08/11/2016
    Representative dot plots of Mas receptor expression in CD34 cells. Right shift indicates the surface expression of Mas receptor
    Mas Antibody - BSA Free NLS1531

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Protocols

View specific protocols for Mas Antibody - BSA Free (NLS1531):

Mas Antibody:
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4-um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene approximately 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol approximately 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol approximately 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol approximately 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water approximately 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block approximately 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody approximately 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T approximately 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary approximately 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T approximately 1 minute at RT.
17. Apply an alkaline phosphatase steptavidin approximately 30 minutes at RT.
18. Rinse the slide in 1X TBS-T approximately 1 minute at RT.
19. Apply an alkaline phosphatase chromagen substrate approximately 30 minutes at RT.
20. Rinse the slide in distilled water approximately 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol approximately 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol approximately 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol approximately 1 minute each @ RT.
24. Wash the slides in 3 changes of xyleneapproximately 1 minute each @ RT.
25. Apply cover slip.

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