MAST205 Antibody (OTI3A5)
Novus Biologicals | Catalog # NBP2-45756
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3A5
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 1482-1798 of human MAST2(NP_055927) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
196.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MAST205 Antibody (OTI3A5)
Western Blot: MAST205 Antibody (OTI3A5) [NBP2-45756]
Western Blot: MAST205 Antibody (OTI3A5) [NBP2-45756] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and MAST2-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with anti-MAST2 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.Western Blot: MAST205 Antibody (OTI3A5) [NBP2-45756]
Western Blot: MAST205 Antibody (OTI3A5) [NBP2-45756] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAST2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAST2.Applications for MAST205 Antibody (OTI3A5)
Application
Recommended Usage
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MAST205
Alternate Names
EC 2.7.11, EC 2.7.11.1, FLJ39200, KIAA0807RP4-533D7.1, MAST205microtubule associated testis specific serine/threonine protein kinase, microtubule associated serine/threonine kinase 2, microtubule-associated serine/threonine-protein kinase 2, MTSSK
Gene Symbol
MAST2
Additional MAST205 Products
Product Documents for MAST205 Antibody (OTI3A5)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MAST205 Antibody (OTI3A5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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