MBD4 Antibody

Novus Biologicals | Catalog # NBP2-24393

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all MBD4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 268-300 and 337-374 of human MBD4.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 17546630).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MBD4 Antibody

Western Blot: MBD4 Antibody [NBP2-24393]

Western Blot: MBD4 Antibody [NBP2-24393]

Western Blot: MBD4 Antibody [NBP2-24393] - analysis of MBD4 in Jurkat cell lysate (30ug) using anti-MBD4 antibody followed by goat anti-rabbit HRP conjugated secondary antibody (Cat. #NB7160). Image from verified customer review.
Western Blot: MBD4 Antibody [NBP2-24393]

Western Blot: MBD4 Antibody [NBP2-24393]

Western Blot: MBD4 Antibody [NBP2-24393] - Analysis of MBD4 in HL60 cell lysate probed with MBD4 antibody.

Applications for MBD4 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation

reported in scientific literature (PMID 15689352)

Western Blot

2-4 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MBD4

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition, MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further, MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI), a form of genomic instability associated with defective DNA mismatch repair, and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene. [provided by RefSeq]

Long Name

Methyl-CpG Binding Domain Protein 4

Alternate Names

EC 3.2.2.-, G/T mismatch glycosylase, G/U mismatch glycosylase, MED1G/5-fluorouracil mismatch glycosylase with biphasic kinetics, methyl-CpG binding domain protein 4, methyl-CpG-binding domain protein 43,N(4)-ethenocytosine glycosylase, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase, putative methyl-CpG binding protein

Gene Symbol

MBD4

Additional MBD4 Products

Product Documents for MBD4 Antibody

Certificate of Analysis

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Product Specific Notices for MBD4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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