Mannan binding lectin (MBL) belongs to the collectin family of innate immune defense proteins, which bind an array of carbohydrate patterns on pathogen surfaces. Collectin family members share several common structural features including a N-terminal, cysteine-rich domain, a collagen-like region, an alpha-helical coiled-coil neck domain and a C-terminal, C-type (Ca2+-dependent ) lectin or carbohydrate recognition domain. MBL homotrimerizes to form a structural unit joined by N-terminal disulfide bonds. These homotrimers further associate into oligomeric structures of up to six units.
Rat MBL-2 ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP2-81169
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Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma, cell supernatant, and other biological fluids (100 ul)
Sensitivity
18.75 pg/mL
Assay Range
31.25 - 2000 pg/mL
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Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Species
Rat
Specificity
This kit recognizes Rat MBL-2 in samples. No significant cross-reactivity or interference between Rat MBL-2 and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% 4.99%
Inter-Assay Precision (Precision between assays) CV% 5.51%
Recovery for Rat MBL-2 ELISA Kit (Colorimetric)
Recovery
88-102%
Linearity
Scientific Data Images for Rat MBL-2 ELISA Kit (Colorimetric)
ELISA: Rat MBL-2 ELISA Kit (Colorimetric) [NBP2-81169] -
ELISA: Rat MBL-2 ELISA Kit (Colorimetric) [NBP2-81169] - Standard Curve ReferenceKit Contents for Rat MBL-2 ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: MBL-2
Long Name
Mannose Binding Lectin 2
Alternate Names
L-MBP, MBL-C
Gene Symbol
Mbl2
Additional MBL-2 Products
Product Documents for Rat MBL-2 ELISA Kit (Colorimetric)
Product Specific Notices for Rat MBL-2 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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