MCM3 Antibody (S04-6B9)

Name: MCM3 Antibody (S04-6B9)
Brand: Novus Biologicals
SKU: NBP3-19485
Price: 559 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-19485

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S04-6B9 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide of human MCM3 (Uniprot # P25205)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MCM3 Antibody (S04-6B9)

Western Blot: MCM3 Antibody (S04-6B9) [NBP3-19485]

Western Blot: MCM3 Antibody (S04-6B9) [NBP3-19485] - Western blot detection of MCM3 in Jurkat, C6, Hela cell lysates using NBP3-19485 (1:1000 diluted). Predicted band size: 91kDa. Observed band size: 100kDa.

Immunohistochemistry-Paraffin: MCM3 Antibody (S04-6B9) [NBP3-19485]

Immunohistochemistry-Paraffin: MCM3 Antibody (S04-6B9) [NBP3-19485] - Immunohistochemistry of MCM3 in paraffin-embedded Human tonsil using NBP3-19485 at dilution 1/1

Immunocytochemistry/Immunofluorescence: MCM3 Antibody (S04-6B9) [NBP3-19485] -

Immunocytochemistry/Immunofluorescence: MCM3 Antibody (S04-6B9) [NBP3-19485] - Immunofluorescence of MCM3 (green) in Hela cells using NBP3-19485 at dilution 1/200, and DAPI (blue)

Applications for MCM3 Antibody (S04-6B9)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/20-1/50

Immunohistochemistry

1/20-1/100

Western Blot

1/1000-1/5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MCM3

MCM3 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

Alternate Names

cervical cancer proto-oncogene 5, DNA polymerase alpha holoenzyme-associated protein P1, DNA replication factor MCM3, DNA replication licensing factor MCM3, EC 3.6.4.12, HCC5, hRlf beta subunit, MCM3 minichromosome maintenance deficient 3, MCM3 minichromosome maintenance deficient 3 (S. cerevisiae), MGC1157, minichromosome maintenance complex component 3, minichromosome maintenance deficient (S. cerevisiae) 3, minichromosome maintenance deficient 3, P1.h, p102, P1-MCM3, replication licensing factor, beta subunit, RLF subunit beta, RLFB

Gene Symbol

MCM3

Additional MCM3 Products

Product Documents for MCM3 Antibody (S04-6B9)

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Certificate of Analysis

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Product Specific Notices for MCM3 Antibody (S04-6B9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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