![Western Blot: MCM6 Antibody [NB100-251]](https://resources.rndsystems.com/images/products/MCM6-Antibody-Western-Blot-NB100-251-img0066.jpg "Western Blot: MCM6 Antibody [NB100-251]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
BSA Free
Loading...
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 775 and the C-terminus (residue 821) of human Minichromosome Maintenance 6 using the numbering given in entry NP_005906.2 (GeneID 4175).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for MCM6 Antibody - BSA Free
Western Blot: MCM6 Antibody [NB100-251]
Western Blot: MCM6 Antibody [NB100-251] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MCM6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry: MCM6 Antibody [NB100-251]](https://resources.rndsystems.com/images/products/MCM6-Antibody-Immunohistochemistry-NB100-251-img0068.jpg "Immunohistochemistry: MCM6 Antibody [NB100-251]")
Immunohistochemistry: MCM6 Antibody [NB100-251]
Immunohistochemistry: MCM6 Antibody [NB100-251] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified goat anti-MCM6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Western Blot: MCM6 Antibody [NB100-251]](https://resources.rndsystems.com/images/products/MCM6-Antibody-Western-Blot-NB100-251-img0002.jpg "Western Blot: MCM6 Antibody [NB100-251]")
Western Blot: MCM6 Antibody [NB100-251]
Western Blot: MCM6 Antibody [NB100-251] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.Applications for MCM6 Antibody - BSA Free
Application
Recommended Usage
Western Blot
1:2000-1:10000
Application Notes
By Western blot, this antibody recognizes bands around 115 kDa representing MCM6.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MCM6
Alternate Names
DNA replication licensing factor MCM6, EC 3.6.4.12, MCG40308, MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe), MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S.cerevisiae), minichromosome maintenance complex component 6, minichromosome maintenance deficient (mis5, S. pombe) 6, minichromosome maintenance deficient 6 homolog, minichromosome maintenance deficient 6 homolog (S. cerevisiae), Mis5, MIS5 homolog, p105MCM
Entrez Gene IDs
4175 (Human)
Gene Symbol
MCM6
UniProt
Additional MCM6 Products
Product Documents for MCM6 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MCM6 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for MCM6 Antibody - BSA Free
There are currently no reviews for this product. Be the first to review MCM6 Antibody - BSA Free and earn rewards!
Have you used MCM6 Antibody - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...