MDC1 Antibody (P2B11) - BSA Free

Novus Biologicals | Catalog # NBP2-12890

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Non-human Primate

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # P2B11

Format

BSA Free
View all MDC1 Primary Antibodies »

Product Specifications

Immunogen

GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus

Localization

Nucleus

Specificity

Detects approx 184kDa. This antibody recognizes MDC1 at and around the N-terminus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MDC1 Antibody (P2B11) - BSA Free

Western Blot: MDC1 Antibody (P2B11) [NBP2-12890]

Western Blot: MDC1 Antibody (P2B11) [NBP2-12890]

Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - Western Blot analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 (NBP2-12890). Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody (NBP2-12890) at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.
Immunocytochemistry/ Immunofluorescence: MDC1 Antibody (P2B11) [NBP2-12890]

Immunocytochemistry/ Immunofluorescence: MDC1 Antibody (P2B11) [NBP2-12890]

Immunocytochemistry/Immunofluorescence: MDC1 Antibody (P2B11) [NBP2-12890] - Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.
Western Blot: MDC1 Antibody (P2B11) [NBP2-12890]

Western Blot: MDC1 Antibody (P2B11) [NBP2-12890]

Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - analysis of Human 293Trap cell lysates showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11. Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.

Applications for MDC1 Antibody (P2B11) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Application Notes
0.5 ug/ml of MDC1 Antibody was sufficient for detection of MDC1 in 10 ug of HeLa cell lysate by ECL immunoblot analysis.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MDC1

0.5 ug/mL was sufficient for detection of MDC1 in 10ug of HeLa cell lysate by ECL immunoblot analysis.

Long Name

Mediator of DNA-damage checkpoint 1

Alternate Names

NFBD1

Gene Symbol

MDC1

Additional MDC1 Products

Product Documents for MDC1 Antibody (P2B11) - BSA Free

Certificate of Analysis

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Product Specific Notices for MDC1 Antibody (P2B11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MDC1 Antibody (P2B11) - BSA Free

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Protocols

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