MEK5 Antibody (7B7O10)

Novus Biologicals | Catalog # NBP3-15451

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7B7O10 expressed in HEK293
View all MEK5 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human MEK5 (Q13163). DVYRKMPEHVLGRIAVAVVKGLTYLWSLKILHRDVKPSNMLVNTRGQVKLCDFGVSTQLVNSIAKTYVGTNAYMAPERISGEQYGIHSDVWSLGISFMELA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEK5 Antibody (7B7O10)

Western Blot: MEK5 Antibody (7B7O10) [NBP3-15451]

Western Blot: MEK5 Antibody (7B7O10) [NBP3-15451]

Western Blot: MEK5 Antibody (7B7O10) [NBP3-15451] - Western blot analysis of extracts of RD cells, using MEK5 Rabbit mAb (NBP3-15451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western Blot: MEK5 Antibody (7B7O10) [NBP3-15451]

Western Blot: MEK5 Antibody (7B7O10) [NBP3-15451]

Western Blot: MEK5 Antibody (7B7O10) [NBP3-15451] - Western blot analysis of extracts of various cell lines, using MEK5 Rabbit mAb (NBP3-15451) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for MEK5 Antibody (7B7O10)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEK5

Mitogen-acitvated protein kinase kinase 5 (MKK5) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). The MAPK cascade plays an important role in signaling defense responses. Of the MKK family members, MKK5 exhibits the closest homology to MEK1 and MEK2, with 46% amino acid identity in the catalytic domain (3) MKK has been show to activate extracellular-signal-regulated protein kinase 5 (ERK5). Also, activated ERK5 phosphorylates mitogen- MKK5 extensively at Ser129, Ser137, Ser142 and Ser149, which are located within the region in MKK5 that is thought to interact with ERK5 (3). There have also been findings to that the MEK5 (MKK5)/ERK5 (BMK) pathway may play a role in prostate cancer (5).

Long Name

Mitogen-activated Protein Kinase Kinase 5

Alternate Names

MAP2K5, MAPKK5, MKK5, PRKMK5

Gene Symbol

MAP2K5

Additional MEK5 Products

Product Documents for MEK5 Antibody (7B7O10)

Certificate of Analysis

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Product Specific Notices for MEK5 Antibody (7B7O10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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