Melan-A/MART-1 Antibody (CL12864) - BSA Free

Novus Biologicals | Catalog # NBP3-18562

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL12864

Format

BSA Free
View all Melan-A/MART-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q16655, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Melan-A/MART-1 Antibody (CL12864) - BSA Free

Western Blot: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562]

Western Blot: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562]

Western Blot: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562] - Analysis in human cell line SK-MEL-30 and human cell line U-2 OS.
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562] - Staining of human prostate shows no positivity in glandular cells as expected.
Immunocytochemistry/ Immunofluorescence: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562]

Immunocytochemistry/ Immunofluorescence: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562]

Immunocytochemistry/Immunofluorescence: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562] - Staining of SK-MEL-30 cells using the Anti-MLANA monoclonal antibody, showing specific staining in endoplasmic reticulum in green. Nuclear probes are visualized in blue (where available).
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562] - Analysis in human skin and prostate tissues using NBP3-18562 antibody. Corresponding MLANA RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562] - Staining of human skin shows strong cytoplasmic positivity in melanocytes.
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562] - Staining of human kidney shows no positivity as expected.
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562]

Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (CL12864) [NBP3-18562] - Staining of human cervix shows no positivity in squamous epithelial cells as expected.

Applications for Melan-A/MART-1 Antibody (CL12864) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. For use with ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Melan-A/MART-1

MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.

Alternate Names

Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA

Gene Symbol

MLANA

Additional Melan-A/MART-1 Products

Product Documents for Melan-A/MART-1 Antibody (CL12864) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Melan-A/MART-1 Antibody (CL12864) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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FAQs

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