MEPCE Antibody

Novus Biologicals | Catalog # NB100-93420

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MEPCE Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence KQQRKFQYGNYCK corresponding to internal region according to NP_062552.2.

Epitope

KQQRKFQYGNYCK

Specificity

Western blot: Preliminary testing was unsuccessful on Human for this particular batch.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MEPCE Antibody

Western Blot: MEPCE Antibody [NB100-93420]

Western Blot: MEPCE Antibody [NB100-93420]

Western Blot: MEPCE Antibody [NB100-93420] - HEK293 overexpressing MEPCE and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: MEPCE Antibody [NB100-93420]

Immunohistochemistry-Paraffin: MEPCE Antibody [NB100-93420]

Immunohistochemistry-Paraffin: MEPCE Antibody [NB100-93420] - Analysis in human Testis shows staining of the spermatids.
Western Blot: MEPCE Antibody [NB100-93420]

Western Blot: MEPCE Antibody [NB100-93420]

Western Blot: MEPCE Antibody [NB100-93420] - (1ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for MEPCE Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Immunohistochemistry-Paraffin

5 - 10 ug/ml

Peptide ELISA

Detection limit 1:2000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 75 kDa band observed in rat testis lysates (calculated MW of 74.4 kDa band according to human NP_062552.2 and 75.3 kDa band according to rat XP_222048.4). In transfected HEK293 transiently expressing human MEPCE a band of approx. 75 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human testis shows staining of the spermatids.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MEPCE

MEPCE belongs to the methyltransferase superfamily and contains one BIN3 domain. It is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it. MEPCE is expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. It is weakly or not expressed in other tissues.

Alternate Names

BCDIN37SK snRNA methylphosphate capping enzyme, bicoid-interacting 3, homolog, Bin3 homolog, bin3, bicoid-interacting 3, homolog (Drosophila), EC 2.1.1.-, FLJ20257, methylphosphate capping enzyme

Entrez Gene IDs

56257 (Human); 231803 (Mouse); 304361 (Rat)

Gene Symbol

MEPCE

UniProt

NP_062552.2 (Human)

Additional MEPCE Products

Product Documents for MEPCE Antibody

Certificate of Analysis

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Product Specific Notices for MEPCE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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