Human MEPE/OF45 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-39809

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.29 ng/mL (example only; lot dependent)

Assay Range

0.937 - 60 ng/mL (example only; lot dependent)
View all MEPE/OF45 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Scientific Data Images for Human MEPE/OF45 ELISA Kit (Colorimetric)

Human MEPE/OF45 ELISA Kit (Colorimetric)

ELISA: Human MEPE/OF45 ELISA Kit (Colorimetric) [NBP3-39809]

ELISA: Human MEPE/OF45 ELISA Kit (Colorimetric) [NBP3-39809] - Standard Curve Reference

Kit Contents for Human MEPE/OF45 ELISA Kit (Colorimetric)

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MEPE/OF45

MEPE, known as OF45 in mouse and rat, is a 55 kDa member of the SIBLING protein family that is primarily expressed in bone and dentin where it regulates the mineralization of those tissues. MEPE is secreted by osteoblasts and dental pulp stem cells during the mineralization process and also by non-mineralizing tissues including epithelial cells in the renal proximal tubule and salivary duct. MEPE contains multiple consensus sites for post-translational modifications, including N-linked glycosylation, N-myristoylation, glycosaminoglycan attachment, and phosphorylation by a variety of kinases. MEPE also contains several putative proteolytic cleavage sites and one integrin-binding RGD motif.

Long Name

Matrix Extracellular Phosphoglycoprotein with ASARM Motif

Alternate Names

OF45, Osteoregulin

Gene Symbol

MEPE

Additional MEPE/OF45 Products

Product Documents for Human MEPE/OF45 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human MEPE/OF45 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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