Human MGMT ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60580

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell Culture (50 uL)

Sensitivity

4.5 ng/mL (example only; lot dependent)

Assay Range

15.625 - 1000 ng/mL (example only; lot dependent)
View all MGMT ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.

Precision

Intra-Assay Precision (Precision within an assay) %CV 6.1% (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 11.2% (example only; lot dependent)

Recovery for Human MGMT ELISA Kit (Colorimetric)

Recovery

89 - 117% (example only; lot dependent)

Scientific Data Images for Human MGMT ELISA Kit (Colorimetric)

ELISA: Human MGMT ELISA Kit (Colorimetric) [NBP2-60580]

ELISA: Human MGMT ELISA Kit (Colorimetric) [NBP2-60580]

ELISA: Human MGMT ELISA Kit (Colorimetric) [NBP2-60580] - Standard curve.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MGMT

O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytotoxic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. In addition, MGMT activity varies among groups of tumors and within a particular type of tumor.

MGMT antibodes are useful tools for DNA repair studies and for some cancer research.

Long Name

O-6 Methylguanine-DNA Methyltransferase

Alternate Names

6-O-methylguanine-DNA methyltransferase, EC 2.1.1.63, methylated-DNA--protein-cysteine methyltransferase, methylguanine-DNA methyltransferase, O-6-methylguanine-DNA methyltransferase, O6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase

Gene Symbol

MGMT

Additional MGMT Products

Product Documents for Human MGMT ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human MGMT ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Cancer Biomarkers
Epigenetics

Customer Reviews for Human MGMT ELISA Kit (Colorimetric) (2)

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  • Human MGMT ELISA Kit (Colorimetric)
    Name: Anonymous
    Sample Tested: Cancer cell lysates
    Verified Customer | Posted 10/14/2022
    The Human MGMT ELISA was used to assess MGMT expression in Temozolomide-Resistant versus parental glioma lines. Results were very consistent.
  • Human MGMT ELISA Kit (Colorimetric)
    Name: MARINA KOUTSIOUMPA
    Sample Tested: Human Glioma cells, whole cell lysates
    Verified Customer | Posted 04/18/2022

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