Recombinant Rat Midkine Protein

Novus Biologicals | Catalog # NBP2-35273

Novus Biologicals
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Key Product Details

Source

E. coli

Applications

Bioactivity, SDS-PAGE
View all Midkine Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 120 amino acids corresponding to Midkine Source: E. coli

Uniprot ID: Q9R1S9

Amino Acid Sequence: VAKKKDKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRIHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK AKAKKGKGKD

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Midkine as determined by LAL method.

Predicted Molecular Mass

13.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Midkine Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat Midkine Protein

SDS-PAGE: Recombinant Rat Midkine Protein [NBP2-35273]

SDS-PAGE: Recombinant Rat Midkine Protein [NBP2-35273]

SDS-Page: Rat Midkine Protein [NBP2-35273]

Formulation, Preparation, and Storage

NBP2-35273
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format Carrier-Free
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Midkine

Midkine, also named MK, MK1, NEGF 2, is belonging to the neurotrophic and developmentally-regulated heparin-binding molecules family and it is encoded by the MDK gene. Midkine includes five intrachain disulfide bonds which held two domains and there are three antiparallel beta-sheets in each domain. A chondroitin sulfate proteoglycan, protein-tyrosine phosphatase zeta (PTPzeta), is a receptor for MK. MK promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis. The predicted molecular weight is approximately 13.2 kDa, based on a mature peptide length of 118 amino acid residues in the mouse and 121 amino acid residues in the human. Mature rat midkine shares 99% and 91% a.a. sequence identity with human and murine midkine, respectively.

Alternate Names

ARAP, MDK, MEK, MK1, NEGF2

Gene Symbol

MDK

Additional Midkine Products

Product Documents for Recombinant Rat Midkine Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Rat Midkine Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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