Mind Bomb 1/MIB1 Antibody (9Q4N9)

Novus Biologicals | Catalog # NBP3-16175

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9Q4N9 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Mind Bomb 1/MIB1 (Q86YT6). MSNSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mind Bomb 1/MIB1 Antibody (9Q4N9)

Western Blot: Mind Bomb 1/MIB1 Antibody (9Q4N9) [NBP3-16175]

Western Blot: Mind Bomb 1/MIB1 Antibody (9Q4N9) [NBP3-16175]

Western Blot: Mind Bomb 1/MIB1 Antibody (9Q4N9) [NBP3-16175] - Western blot analysis of extracts of various cell lines, using Mind Bomb 1/MIB1 Rabbit mAb (NBP3-16175) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for Mind Bomb 1/MIB1 Antibody (9Q4N9)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Mind Bomb 1/MIB1

E3 ubiquitin ligase protein mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. It positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. It probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.

Long Name

Mindbomb Homolog 1

Alternate Names

DIP1, MIB1, ZZANK2

Gene Symbol

MIB1

Additional Mind Bomb 1/MIB1 Products

Product Documents for Mind Bomb 1/MIB1 Antibody (9Q4N9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mind Bomb 1/MIB1 Antibody (9Q4N9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail