MIPOL1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86161

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MIPOL1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KTAALVEEVYFAQKERDEAVMSRLQLAIEERDEAIARAKHMEMSLKVLENINPEENDMTLQELLNRINNADTGIAIQKNGAIIVDRIYKTKECKMRITAEEMSALIEERDAALSKCKRLEQELHHVKEQNQTSANNMRHLTAENNQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MIPOL1 Antibody - BSA Free

Immunohistochemistry-Paraffin: MIPOL1 Antibody [NBP1-86161]

Immunohistochemistry-Paraffin: MIPOL1 Antibody [NBP1-86161]

Immunohistochemistry-Paraffin: MIPOL1 Antibody [NBP1-86161] - Staining in human prostate and skeletal muscle tissues using anti-MIPOL1 antibody. Corresponding MIPOL1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MIPOL1 Antibody [NBP1-86161]

Immunohistochemistry-Paraffin: MIPOL1 Antibody [NBP1-86161]

Immunohistochemistry-Paraffin: MIPOL1 Antibody [NBP1-86161] - Staining of human prostate shows high expression.
Immunohistochemistry-Paraffin: MIPOL1 Antibody [NBP1-86161]

Immunohistochemistry-Paraffin: MIPOL1 Antibody [NBP1-86161]

Immunohistochemistry-Paraffin: MIPOL1 Antibody [NBP1-86161] - Staining of human skeletal muscle shows low expression as expected.
MIPOL1 Antibody - BSA Free Western Blot: MIPOL1 Antibody - BSA Free [NBP1-86161]

Western Blot: MIPOL1 Antibody - BSA Free [NBP1-86161]

Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp

Applications for MIPOL1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MIPOL1

MIPOL1, also known as Mirror-image polydactyly gene 1 protein, has 3 isoforms, a 442 amino acid isoform1 that is 51 kDa, a 261 amino acid isoform 2 that is 30 kDa and a short 169 amino acid isoform 3 that is 20 kDa; expressed very weakly in heart, liver, skeletal muscle, kidney, pancreas and fetal kidney; and may function as a tumor suppressor. Studies on this protein have shown a relationship with the following diseases and disorders: mirror-image polydactyly, Laurin-Sandrow syndrome, adult astrocytic tumour, fibular hypoplasia, intrahepatic cholangiocarcinoma, nasopharyngitis and carcinoma. This protein has shown an interaction with UBC protein.

Alternate Names

DKFZp313M2036, MGC34010, mirror-image polydactyly 1, mirror-image polydactyly gene 1 protein

Gene Symbol

MIPOL1

Additional MIPOL1 Products

Product Documents for MIPOL1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MIPOL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MIPOL1 Antibody - BSA Free

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Protocols

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