Mithramycin A

Tocris Bioscience | Catalog # 1489

Inhibitor of DNA and RNA polymerase
Tocris Bioscience

Key Product Details

Description

Inhibitor of DNA and RNA polymerase
View all RNA/DNA Polymerase Inhibitors »

Product Description

Mithramycin A is an anticancer antibiotic that selectively binds to G-C-rich DNA in the presence of Mg2+ or Zn2+, inhibiting RNA and DNA polymerase action. Inhibits c-myc expression and induces myeloid differentiation of HL-60 promyelocytic leukemia cells.

Product Specifications for Mithramycin A

Molecular Weight

1085.16

Formula

C52H76O24

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

(1S)-5-Deoxy-1-C-[(2S,3S)-7-[[2,6-dideoxy-3-O-(2,6-dideoxy-β-D-arabino-hexopyranosyl)-β-D-arabino-hexopyranosyl]oxy]-3-[(O-2,6-dideoxy-3-C-methyl-β-D-ribo-hexopyranosyl-(1.fwdarw.3)-O-2,6-dideoxy-β-D-lyxo-hexopyranosyl-(1.fwdarw.3)-2,6-dideoxy-β-D-arabino-hexopyranosyl)oxy]-1,2,3,4-tetrahydro-5,10-dihydroxy-6-methyl-4-oxo-2-anthracenyl]-1-O-methyl-D-threo-2-pentulose

CAS Number

18378-89-7

PubChem ID

90479798

InChI Key

CFCUWKMKBJTWLW-YZCDWGPJSA-N

SMILES

OC1=C2C(C=C3C(C([C@@H](OC5OC(C)C(O)C(OC6OC(C)C(O)C(OC7OC(C)C(O)C(O)(C)C7)C6)C5)[C@@]([C@H](OC)C([C@@H](O)[C@H](O)C)=O)([H])C3)=O)=C2O)=CC(O[C@@H]4OC(C)C(O)C(OC8OC(C)C(O)C(O)C8)C4)=C1C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 54.26 50

Preparing Stock Solutions for Mithramycin A

The following data is based on the product molecular weight 1085.16.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.5 mM 1.84 mL 9.22 mL 18.43 mL
2.5 mM 0.37 mL 1.84 mL 3.69 mL
5 mM 0.18 mL 0.92 mL 1.84 mL
25 mM 0.04 mL 0.18 mL 0.37 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 21 publications citing the usage of this product.

Product Documents for Mithramycin A

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mithramycin A

For research use only

⚠ WARNING: This product can expose you to chemicals including Plicamycin, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Epigenetics

Customer Reviews for Mithramycin A (1)

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  • Mithramycin A completely kills melanoma cell line at micromolar concentrations
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: SK-MEL-28
    Verified Customer | Posted 02/07/2019
    Used for killing assay for in vitro cell culture. Cells were cultured for 24 hours before the addition of the compound into the media at low micromolar concentrations (indicated by the arrow).
    Mithramycin A 1489

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