Mitofusin 1 Antibody (11G8) - BSA Free

Novus Biologicals | Catalog # NBP3-26143

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 11G8 expressed in HEK293

Format

BSA Free
View all Mitofusin 1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Mitofusin 1 [UniProt Q8IWA4]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mitofusin 1 Antibody (11G8) - BSA Free

Mitofusin 1 Antibody (11G8)

Flow Cytometry: Mitofusin 1 Antibody (11G8) [NBP3-26143] -

Flow Cytometry: Mitofusin 1 Antibody (11G8) [NBP3-26143] - Overlay Peak curve showing HepG2 cells stained with Mitofusin 1 Antibody (11G8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Mitofusin 1 Antibody (11G8) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Mitofusin 1

MFN1 (Mitofusin-1) is a nuclear encoded mitochondrial membrane protein that mediates mitochondrial fusion and morphology in mammalian cells. MFN1 and MFN2 are homologs of the Drosophila protein Fuzzy Onion (Fzo) that interact with each other to facilitate mitochondrial targeting. MFN1 is ubiquitously expressed,with slightly higher leves detectedl in kidney and heart. MFN2 may be overexpressed in some tumors, such as lung cancers.

Mitochondrial fusion occurs in many cell types and is a fundamental component of mitochondria morphology, which is balanced between fusion and fission. Mitochondrial dysfunction is a major cause of neuronal death in degenerative disorders, such as Parkinson's disease, and can be triggered by an imbalance in the dynamic equilibrium between mitochondrial fission and fusion.

Alternate Names

hfzo1, hfzo2, MFN1

Gene Symbol

MFN1

Additional Mitofusin 1 Products

Product Documents for Mitofusin 1 Antibody (11G8) - BSA Free

Certificate of Analysis

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Product Specific Notices for Mitofusin 1 Antibody (11G8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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Protocols

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