MitoPY1

Tocris Bioscience | Catalog # 4428

Fluorescent mitochondrial hydrogen peroxide indicator. Used for: live cell imaging. Application: confocal microscopy
Tocris Bioscience

Key Product Details

Description

Fluorescent mitochondrial hydrogen peroxide indicator. Used for: live cell imaging. Application: confocal microscopy

Wavelength

Excitation = 510 nm, Emission = 530 nm
View all Fluorescent Cell Indicators and Sensors »

Product Description

Key information: mitochondria peroxy yellow 1 (MitoPY1) is a fluorescent probe for imaging hydrogen peroxide (H2O2) in mitochondria of living cells.

Used for: imaging hydrogen peroxide (H2O2) in mitochondria of living cells. Can also detect local increases in H2O2 in an in vitro model of Parkinson's Disease.

Application: confocal microscopy.

Properties and Photophysical Data: selective for H2O2 over superoxide, nitric oxide and hydroxyl radical.

Excitation and emission maxima (λ) are 510 nm and 530 nm, respectively, quantum yield = 0.405.

Licensing Information

Sold under license from the Regents of the University of California

Optical Data

λabs 510 nm
λem 530 nm
Quantum Yield (φ) 0.405
Application Confocal Imaging

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Product Specifications for MitoPY1

Molecular Weight

954.68

Formula

C52H53BIN2O5P

Storage

Store at -20°C

Chemical Name

[4-[4-[3-Oxo-6'-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl]-1-piperazinyl]butyl]triphenyl-phosphonium iodide

CAS Number

1041634-69-8

PubChem ID

90488942

InChI Key

JPCCULDYUUMFAD-UHFFFAOYSA-M

SMILES

O=C(O4)C5=C(C=CC=C5)C24C1=CC=C(N6CCN(CCCC[P+](C9=CC=CC=C9)(C%10=CC=CC=C%10)C8=CC=CC=C8)CC6)C=C1OC3=C2C=CC(B7OC(C)(C)C(C)(C)O7)=C3.[I-]

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 95.47 100

Preparing Stock Solutions for MitoPY1

The following data is based on the product molecular weight 954.68.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.05 mL 5.24 mL 10.47 mL
5 mM 0.21 mL 1.05 mL 2.09 mL
10 mM 0.10 mL 0.52 mL 1.05 mL
50 mM 0.02 mL 0.10 mL 0.21 mL

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 84 publications citing the usage of this product.

Product Documents for MitoPY1

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MitoPY1

For research use only

Customer Reviews for MitoPY1 (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used MitoPY1?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Cells were incubated in the well with 8 μg/ml MitoPy diluted in PBS
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Fibroblasts
    Verified Customer | Posted 12/10/2019
    Cells were incubated in the well with 8 μg/ml MitoPy diluted in PBS
    MitoPY1 4428

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All