MKK3/MKK6 Antibody (8R1J5)

Novus Biologicals | Catalog # NBP3-15946

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8R1J5 expressed in HEK293
View all MKK3/MKK6 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 248-347 of human MKK3/MKK6 (P46734). KSDVWSLGITMIEMAILRFPYESWGTPFQQLKQVVEEPSPQLPADRFSPEFVDFTAQCLRKNPAERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MKK3/MKK6 Antibody (8R1J5)

Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946]

Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946]

Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946] - Western blot analysis of extracts of various cell lines, using MKK3/MKK6 Rabbit mAb (NBP3-15946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946]

Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946]

Western Blot: MKK3/MKK6 Antibody (8R1J5) [NBP3-15946] - Western blot analysis of extracts of various cell lines, using MKK3/MKK6 Rabbit mAb (NBP3-15946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for MKK3/MKK6 Antibody (8R1J5)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MKK3/MKK6

Mitogen-activated protein kinase kinase 3 (MKK3), also known as MEK3, and the closely related MKK6 are activated by proinflammatory cytokines and environmental stresses. Activation occurs through phosphorylation by several upstream MAPK kinase kinases, including ASK1 and TAK1. Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr.

Long Name

Mitogen-activated Protein Kinase Kinase 3

Alternate Names

Dual specificity mitogen-activated protein kinase kinase 6;MAP2K6;MAPKK6;MEK6;MKK6;PRKMK6;SAPKK3;SAPKK-3

Gene Symbol

MAP2K6

Additional MKK3/MKK6 Products

Product Documents for MKK3/MKK6 Antibody (8R1J5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MKK3/MKK6 Antibody (8R1J5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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