Mouse MMP-9 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60095

Novus Biologicals
Discontinued Product
NBP2-60095 has been discontinued. View all MMP-9 products.

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

0.552 ng/mL

Assay Range

0.625 ng/mL - 40 ng/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Description

This ELISA kit has been transitioned to a new version, available here: MMPT90

Precision

Intra-Assay Precision (Precision within an assay) %CV 8

Inter-Assay Precision (Precision between assays) %CV 10

Scientific Data Images for Mouse MMP-9 ELISA Kit (Colorimetric)

ELISA: Mouse MMP-9 ELISA Kit (Colorimetric) [NBP2-60095]

ELISA: Mouse MMP-9 ELISA Kit (Colorimetric) [NBP2-60095]

ELISA: Mouse MMP-9 ELISA Kit (Colorimetric) [NBP2-60095] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Mouse MMP-9 ELISA Kit (Colorimetric)

Mouse MMP-9 ELISA Kit (Colorimetric) [NBP2-60095] -

Mouse MMP-9 ELISA Kit (Colorimetric) [NBP2-60095] - Standard Curve Reference

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MMP-9

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Gene Symbol

MMP9

Additional MMP-9 Products

Product Documents for Mouse MMP-9 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Mouse MMP-9 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations for Mouse MMP-9 ELISA Kit (Colorimetric)

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FAQs for Mouse MMP-9 ELISA Kit (Colorimetric)

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  • Q:  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 

    A: We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.
    It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.
    6 have been tested for detection, seen here.

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