MMP-9 (Gelatinase B) is a metalloproteinase that degrades multiple extracellular matrix components including Gelatin, Collagens III, IV, V, and XI, Elastin, Nidogen-1, and Vitronectin. It also cleaves a variety of chemokines and growth factors (e.g. IL-1 beta, CXCL8/IL-8, CXCL7, CXCL4, CXCL1, Latent TGF-beta, membrane bound TNF-alpha, VEGF, and FGF basic), Amyloid beta peptide, Substance P, and Myelin Basic Protein. MMP-9 can trigger signaling through various transmembrane proteins or inhibit signaling by inducing their shedding from the cell surface (e.g. CD44, E-Cadherin, Integrins, ICAM-1, and CD25/IL-2 R alpha). The activity of MMP-9 is regulated by its association with the TIMP-1 protease inhibitor. Circulating levels of MMP-9 are elevated in many inflammatory disorders including intraluminal thrombus formation, atherosclerosis, Crohn’s disease, hepatitis C virus infection, colorectal cancer, and Duchenne muscular dystrophy.
Mouse Total MMP-9 Quantikine ELISA Kit
R&D Systems | Catalog # MMPT90
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.014 ng/mL
Assay Range
0.078-5 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
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Product Summary for Mouse Total MMP-9 Quantikine ELISA Kit
The Quantikine Mouse MMP-9 (total) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure total mouse MMP-9 (pro-, active, and TIMP-complexed MMP-9) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse MMP-9 and antibodies raised against the recombinant factor. Natural mouse MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural mouse MMP-9.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse MMP-9 (pro-, active, and TIMP-complexed)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 0.239 | 0.731 | 1.71 | 0.211 | 0.600 | 1.61 |
| Standard Deviation | 0.024 | 0.037 | 0.097 | 0.020 | 0.049 | 0.125 |
| CV% | 8.4 | 5.1 | 5.7 | 9.6 | 8.1 | 7.8 |
Recovery for Mouse Total MMP-9 Quantikine ELISA Kit
The recovery of mouse MMP-9 spiked to 3 levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 94 | 86-108 |
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse MMP-9 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse Total MMP-9 Quantikine ELISA Kit
Mouse Total MMP-9 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-9
Long Name
Matrix Metalloproteinase 9
Alternate Names
CLG4B, Gelatinase B, GELB, MANDP2, MMP9
Gene Symbol
MMP9
Additional MMP-9 Products
Product Documents for Mouse Total MMP-9 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Total MMP-9 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Mouse Total MMP-9 Quantikine ELISA Kit
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Sample Tested: IC-21 mouse macrophage cell lineVerified Customer | Posted 11/15/2019
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Protocols
View specific protocols for Mouse Total MMP-9 Quantikine ELISA Kit (MMPT90):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- How to Run an R&D Systems Quantikine ELISA
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- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
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- Troubleshooting Guide: ELISA
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