Mouse Total MMP-9 Quantikine ELISA Kit

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Mouse Total MMP-9 ELISA Standard Curve
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Product Details
Citations (34)
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Mouse Total MMP-9 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)
0.014 ng/mL
Assay Range
0.1 - 5 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Natural and recombinant mouse MMP-9 (pro-, active, and TIMP-complexed)
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse MMP-9 (total) Immunoassay is a 4.5 hour solid-phase ELISA designed to measure total mouse MMP-9 (pro-, active, and TIMP-complexed MMP-9) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse MMP-9 and antibodies raised against the recombinant factor. Natural mouse MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative levels of natural mouse MMP-9.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 0.239 0.731 1.71 0.211 0.6 1.61
Standard Deviation 0.024 0.037 0.097 0.02 0.049 0.125
CV% 8.4 5.1 5.7 9.6 8.1 7.8


The recovery of mouse MMP-9 spiked to 3 levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 94 86-108


To assess the linearity of the assay, samples containing high concentrations of mouse MMP-9 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse Total MMP-9 ELISA Linearity

Data Examples

Mouse Total MMP-9 ELISA Standard Curve

Product Datasheets

Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MMP-9

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name:
Matrix Metalloproteinase 9
Entrez Gene IDs:
4318 (Human); 17395 (Mouse); 81687 (Rat)
Alternate Names:
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse Total MMP-9 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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  1. Development of Kaempferol-Loaded Gelatin Nanoparticles for the Treatment of Corneal Neovascularization in Mice
    Authors: YL Chuang, HW Fang, A Ajitsaria, KH Chen, CY Su, GS Liu, CL Tseng
    Pharmaceutics, 2019;11(12):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  2. Vesicular nucleotide transporter mediates ATP release and migration in neutrophils
    Authors: Y Harada, Y Kato, T Miyaji, H Omote, Y Moriyama, M Hiasa
    J. Biol. Chem., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Murine neutrophils treated with alphaB-crystallin reduce IL-12p40 production by dendritic cells
    Authors: TM Finlay, AL Palmer, SS Ousman
    Immunology, 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Anti-inflammatory signaling by mammary tumor cells mediates prometastatic macrophage polarization in an innovative intraductal mouse model for triple-negative breast cancer
    Authors: J Steenbrugg, K Breyne, K Demeyere, O De Wever, NN Sanders, W Van Den Br, C Colpaert, P Vermeulen, S Van Laere, E Meyer
    J. Exp. Clin. Cancer Res., 2018;37(1):191.
    Species: Mouse
    Sample Types: Serum
  5. Ginkgo biloba extracts prevent aortic rupture in angiotensin II-infused hypercholesterolemic mice
    Authors: XF Huang, SZ Zhang, YY You, N Zhang, H Lu, A Daugherty, XJ Xie
    Acta Pharmacol. Sin., 2018;0(0):.
    Species: Mouse
    Sample Types: Serum
  6. Fungal immunomodulatory protein-fve could modulate airway remodel through by affect IL17 cytokine
    Authors: YT Lee, CT Wu, HL Sun, JL Ko, KH Lue
    J Microbiol Immunol Infect, 2017;0(0):.
    Species: Mouse
    Sample Types: BALF
  7. CCAAT/enhancer-binding protein ? overexpression alleviates myocardial remodelling by regulating angiotensin-converting enzyme-2 expression in diabetes
    Authors: Y Tie, C Zhai, Y Zhang, X Qin, F Yu, H Li, M Shan, C Zhang
    J. Cell. Mol. Med., 2017;0(0):.
    Species: Mouse
    Sample Types: Serum
  8. Adipose progenitor cell secretion of GM-CSF and MMP9 promotes a stromal and immunological microenvironment that supports breast cancer progression
    Authors: F Reggiani, V Labanca, P Mancuso, C Rabascio, G Talarico, S Orecchioni, A Manconi, F Bertolini
    Cancer Res., 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. Zinc metalloproteinase ZmpC suppresses experimental pneumococcal meningitis by inhibiting bacterial invasion of central nervous systems
    Authors: M Yamaguchi, M Nakata, R Sumioka, Y Hirose, S Wada, Y Akeda, T Sumitomo, S Kawabata
    Virulence, 2017;0(0):0.
    Species: Mouse
    Sample Types: Plasma
  10. Carvedilol attenuates paraquat-induced lung injury by inhibition of proinflammatory cytokines, chemokine MCP-1, NF-?B activation and oxidative stress mediators
    Cytokine, 2016;88(0):144-153.
    Species: Mouse
    Sample Types: Plasma
  11. SHIP1-expressing mesenchymal stem cells regulate hematopoietic stem cell homeostasis and lineage commitment during aging.
    Authors: Iyer S, Brooks R, Gumbleton M, Kerr W
    Stem Cells Dev, 2015;24(9):1073-81.
    Species: Mouse
    Sample Types: Serum
  12. beta-Glucuronidase, a Regulator of Lyme Arthritis Severity, Modulates Lysosomal Trafficking and MMP-9 Secretion in Response to Inflammatory Stimuli.
    Authors: Bramwell K, Mock K, Ma Y, Weis J, Teuscher C, Weis J
    J Immunol, 2015;195(4):1647-56.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. Eosinophils promote generation and maintenance of immunoglobulin-A-expressing plasma cells and contribute to gut immune homeostasis.
    Authors: Chu V, Beller A, Rausch S, Strandmark J, Zanker M, Arbach O, Kruglov A, Berek C
    Immunity, 2014;40(4):582-93.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. Soluble E-cadherin: a critical oncogene modulating receptor tyrosine kinases, MAPK and PI3K/Akt/mTOR signaling.
    Authors: Brouxhon, S M, Kyrkanides, S, Teng, X, Athar, M, Ghazizadeh, S, Simon, M, O'Banion, M K, Ma, L
    Oncogene, 2014;33(2):225-35.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Activation of transcription factor Nrf2 signalling by the sphingosine kinase inhibitor SKI-II is mediated by the formation of Keap1 dimers.
    Authors: Mercado, Nicolas, Kizawa, Yasuo, Ueda, Keitaro, Xiong, Yeping, Kimura, Genki, Moses, Audric, Curtis, Jonathan, Ito, Kazuhiro, Barnes, Peter J
    PLoS ONE, 2014;9(2):e88168.
    Species: Mouse
    Sample Types: BALF
  16. 12-Hydroxyheptadecatrienoic acid promotes epidermal wound healing by accelerating keratinocyte migration via the BLT2 receptor.
    Authors: Liu M, Saeki K, Matsunobu T, Okuno T, Koga T, Sugimoto Y, Yokoyama C, Nakamizo S, Kabashima K, Narumiya S, Shimizu T, Yokomizo T
    J Exp Med, 2014;211(6):1063-78.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  17. Pharmacologic inhibition of S-nitrosoglutathione reductase protects against experimental asthma in BALB/c mice through attenuation of both bronchoconstriction and inflammation.
    Authors: Blonder J, Mutka S, Sun X, Qiu J, Green L, Mehra N, Boyanapalli R, Suniga M, Look K, Delany C, Richards J, Looker D, Scoggin C, Rosenthal G
    BMC Pulm Med, 2014;14(0):3.
    Species: Mouse
    Sample Types: Plasma
  18. Notch-1 mediated cardiac protection following embryonic and induced pluripotent stem cell transplantation in doxorubicin-induced heart failure.
    Authors: Merino H, Singla D
    PLoS ONE, 2014;9(7):e101024.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. A novel molecule Me6TREN promotes angiogenesis via enhancing endothelial progenitor cell mobilization and recruitment.
    Authors: Chen H, Wang S, Zhang J, Ren X, Zhang R, Shi W, Lv Y, Zhou Y, Yan X, Chen L, He L, Zhang B, Nan X, Yue W, Li Y, Pei X
    Sci Rep, 2014;4(0):6222.
  20. The combination of the histone deacetylase inhibitor vorinostat and synthetic triterpenoids reduces tumorigenesis in mouse models of cancer.
    Authors: Tran, Kim, Risingsong, Renee, Royce, Darlene, Williams, Charlott, Sporn, Michael, Pioli, Patricia, Gediya, Lalji K, Njar, Vincent, Liby, Karen T
    Carcinogenesis, 2013;34(1):199-210.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. Tissue redox activity as a hallmark of carcinogenesis: from early to terminal stages of cancer.
    Authors: Bakalova R, Zhelev Z, Aoki I, Saga T
    Clin Cancer Res, 2013;19(9):2503-17.
    Species: Mouse
    Sample Types: Plasma
  22. Role of c-Jun N-terminal protein kinase 1/2 (JNK1/2) in macrophage-mediated MMP-9 production in response to Moraxella catarrhalis lipooligosaccharide (LOS).
    Authors: Hassan F, Ren D, Zhang W
    PLoS ONE, 2012;7(5):e37912.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. A hypomorphic mutation in the Gfi1 transcriptional repressor results in a novel form of neutropenia.
    Eur J Immunol, 2012;42(9):2395-408.
    Species: Mouse
    Sample Types: BALF
  24. The effects of low levels of dystrophin on mouse muscle function and pathology.
    Authors: van Putten M, Hulsker M, Nadarajah VD, van Heiningen SH, van Huizen E, van Iterson M, Admiraal P, Messemaker T, den Dunnen JT, 't Hoen PA, Aartsma-Rus A
    PLoS ONE, 2012;7(2):e31937.
    Species: Mouse
    Sample Types: Serum
  25. The synthetic triterpenoid CDDO-methyl ester delays estrogen receptor-negative mammary carcinogenesis in polyoma middle T mice.
    Authors: Tran K, Risingsong R, Royce D
    Cancer Prev Res (Phila), 2012;5(5):726-34.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  26. Activation of matrix metalloproteinases following anti-Abeta immunotherapy; implications for microhemorrhage occurrence.
    Authors: Wilcock DM, Morgan D, Gordon MN, Taylor TL, Ridnour LA, Wink DA, Colton CA
    J Neuroinflammation, 2011;8(0):115.
    Species: Mouse
    Sample Types: Tissue Homogenates
  27. Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5.
    Authors: Conant K, Lonskaya I, Szklarczyk A, Krall C, Steiner J, Maguire-Zeiss K, Lim ST
    J. Neurochem., 2011;118(4):521-32.
    Species: Mouse
    Sample Types: Cell Lysates
  28. Early protection from burn-induced acute lung injury by deletion of preprotachykinin-A gene.
    Authors: Sio SW, Moochhala S, Lu J
    Am. J. Respir. Crit. Care Med., 2010;181(1):36-46.
    Species: Mouse
    Sample Types: Tissue Homogenates
  29. Galectin-3 contributes to neonatal hypoxic-ischemic brain injury.
    Authors: Doverhag C, Hedtjarn M, Poirier F, Mallard C, Hagberg H, Karlsson A, Savman K
    Neurobiol. Dis., 2010;38(1):36-46.
    Species: Mouse
    Sample Types: Tissue Homogenates
  30. 15-Epi-lipoxin A(4) inhibits the progression of endometriosis in a murine model.
    Authors: Chen QH, Zhou WD, Pu DM, Huang QS, Li T, Chen QX
    Fertil. Steril., 2010;93(5):1440-7.
    Species: Mouse
    Sample Types: Peritoneal Fluid
  31. Inhibition of EGFR pathway signaling and the metastatic potential of breast cancer cells by PA-MSHA mediated by type 1 fimbriae via a mannose-dependent manner.
    Authors: Liu ZB, Hou YF, Zhu J
    Oncogene, 2010;29(20):2996-3009.
    Species: Mouse
    Sample Types: Serum
  32. Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.
    Authors: Koronyo-Hamaoui M, Ko MK, Koronyo Y, Azoulay D, Seksenyan A, Kunis G, Pham M, Bakhsheshian J, Rogeri P, Black KL, Farkas DL, Schwartz M
    J. Neurochem., 2009;111(6):1409-24.
    Species: Mouse
    Sample Types: Tissue Homogenates
  33. Inducible nitric oxide synthase deficiency impairs matrix metalloproteinase-9 activity and disrupts leukocyte migration in hepatic ischemia/reperfusion injury.
    Authors: Hamada T, Duarte S, Tsuchihashi S, Busuttil RW, Coito AJ
    Am. J. Pathol., 2009;174(6):2265-77.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  34. Matrix metalloproteinase 9 facilitates West Nile virus entry into the brain.
    Authors: Wang P, Dai J, Bai F, Kong KF, Wong SJ, Montgomery RR, Madri JA, Fikrig E
    J. Virol., 2008;82(18):8978-85.
    Species: Mouse
    Sample Types: Serum


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Mouse Total MMP-9 Quantikine ELISA Kit
By Anonymous on 11/15/2019
Application: Sample Tested: IC-21 mouse macrophage cell line

Mouse Total MMP-9 Quantikine ELISA Kit
By Anonymous on 05/02/2017
Application: Sample Tested: Lung tissue