Bovine MMP-9 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-42428

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Tissue homogenatesand other biological fluids (100 uL)

Sensitivity

0.123 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Bovine

Description

Assay time: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Recovery for Bovine MMP-9 ELISA Kit (Colorimetric)

Recovery

80 - 99% (example only; lot dependent)

Kit Contents for Bovine MMP-9 ELISA Kit (Colorimetric)

  • Assay Diluent A
  • Assay Diluent B
  • Detection Reagent A
  • Detection Reagent B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated ready to use 96-well strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: MMP-9

Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that degrade substances within the extracellular matrix. The MMP family includes six different groups of enzymes: collagenases, gelatinases, stromelysins, transmembrane MMPs, matrilysins and others. MMPs are secreted as proenzymes that have to be cleaved in order to be activated. Other MMPs, plasmins as well as other factors activate MMPs. MMPs are thought to play an important role in tissue remodeling associated with various physiological and pathological processes. MMP9 degrades of proteins in the extracellular matrix and activates growth factors like proTGF beta and proTNF alpha. MMP9 contributes to the invasion and metastasis of various human malignancies.

Long Name

Matrix Metalloproteinase 9

Alternate Names

CLG4B, Gelatinase B, GELB, MANDP2, MMP9

Gene Symbol

MMP9

Additional MMP-9 Products

Product Documents for Bovine MMP-9 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Bovine MMP-9 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Bovine MMP-9 ELISA Kit (Colorimetric)

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  • Q:  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 

    A: We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.
    It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.
    6 have been tested for detection, seen here.

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