Mouse Angiopoietin‑like Protein 4/ANGPTL4 Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # FAB97971AFP680

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

ELISA

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 547113
View all Angiopoietin-like Protein 4/ANGPTL4 Primary Antibodies »

Product Specifications

Specificity

Detects mouse Angiopoietin-like Protein 4/ANGPTL4 in direct Elisa.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications

Application
Recommended Usage

ELISA

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Angiopoietin-like Protein 4/ANGPTL4

Angiopoietin-like 4 (ANGPTL4), also known as FIAF, FARP, and PGAR, is a 55 kDa glycoprotein secreted by the liver and fat tissue. It is structurally related to the angoipoietins and contains an N-terminal coiled coil domain and a C-terminal fibrinogen-like domain which can be proteolytically separated in vivo (1). Mature mouse ANGPTL4 shares 26%-30% amino acid (aa) sequence identity with mouse ANGPTL1, 2, 3, 4, 6, and 7. It shares 75% and 97% aa sequence identity with human and rat ANGPTL4, respectively. The coiled coil domain, which is not glycosylated, mediates the formation of variable sized disulfide-linked oligomers (2). This domain directly inhibits lipoprotein lipase, resulting in increased circulating triglyceride levels (3, 4). In humans, the N-terminal fragment and full length ANGPTL4 physically associate with HDL (4). In mouse, however, full length ANGPTL4 associates with HDL, while the N-terminal fragment associates with LDL (4). Circulating ANGPTL4 is decreased in type II diabetics with a subsequent loss of its normal plasma glucose lowering activity (5). Its expression in adipose tissue is induced by fasting and suppressed by feeding (6). In hypoxic areas, ANGPTL4 is induced in both vascular endothelial cells and tumor cells (7, 8). The N-terminal fragment can function as an angiogenesis inhibitor (7, 8). In contrast, the C-terminal fragment modulates cell adhesion through interactions with heparan sulfate proteoglycans, Integrins  beta 1 and beta 5, Vitronectin, and Fibronectin, thereby promoting keratinocyte migration and wound healing (7, 9, 10). ANGPTL4 additionally enhances the survival of hematopoietic and mesenchymal stem cells (11, 12). The expression of an undersialylated form of ANGPTL4 in renal podocytes contributes to proteinuria and nephrotic syndrome (13).

References

  1. Zhu, P. et al. (2012) Biosci. Rep. 32:211.
  2. Ge, H. et al. (2004) J. Biol. Chem. 279:2038.
  3. Sukonina, V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:17450.
  4. Mandard, S. et al. (2006) J. Biol. Chem. 281:934.
  5. Xu, A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6086.
  6. Kersten, S. et al. (2000) J. Biol. Chem. 275:28488.
  7. Cazes, A. et al. (2006) Circ. Res. 99:1207.
  8. Le Jan, S. et al. (2003) Am. J. Pathol. 162:1521.
  9. Goh, Y.Y. et al. (2010) Am. J. Pathol. 177:2791.
  10. Goh, Y.Y. et al. (2010) J. Biol. Chem. 285:32999.
  11. Blank, U. et al. (2012) Eur. J. Haematol. 89:198.
  12. Hou, M. et al. (2014) PLoS ONE 9:e85808.
  13. Clement, L.C. et al. (2011) Nat. Med. 17:117.

Alternate Names

Angiopoietin like Protein 4, ANGPTL4, FIAF, HFARP, PGAR

Entrez Gene IDs

51129 (Human); 57875 (Mouse); 362850 (Rat)

Gene Symbol

ANGPTL4

UniProt

Q9Z1P8

Additional Angiopoietin-like Protein 4/ANGPTL4 Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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