Mouse Autoimmune Regulator/AIRE Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Autoimmune Regulator/AIRE

AIRE (Autoimmune regulator; also APECED) is a 58-60 kDa transcriptional regulator that exhibits tight regulation of its expression. It is widely expressed in lymphoid organs, but is most pronounced in thymic medullary epithelium, where it induces the expression of organ specific antigens that are used to sequester and delete autoreactive lymphocytes. Mouse AIRE is 552 amino acids (aa) in length. It contains multiple domains, including an HSR domain with two LXXLL motifs (aa 1-106), an NLS (aa 114-134), one SAND domain (aa 182-282) and two PHD zinc-finger regions (aa 298-345; 434-475). AIRE will homodimerize and tetramerize. There are multiple potential isoforms that involve various combinations of deletions of aa 265-268, 368-426 and Lys296, plus a 42 aa substitution for aa 368-552. Over aa 476-552, mouse AIRE shares 91% and 65% aa identity with rat and human AIRE, respectively.

Product Datasheets

Related Research Areas

• Transcriptional Regulators in Allergy and Asthma