Mouse B7-H2 APC-conjugated Antibody

FAB158A has been discontinued. View all B7-H2 products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse B7-H2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-H2, rhB7-H3, rhB7-H3b,
    rhPD-L2, recombinant mouse (rm) B7-1, rmB7-2, rmB7-H1, rmB7-H3, rmB7-H4, rmPD-L2, or recombinant rat B7-2 is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 599841
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse B7-H2        
    Glu47-Lys279
    Accession # Q9JHJ8
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of B7‑H2 in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse B7‑H2 APC‑conjugated Monoclonal Antibody (Catalog # FAB158A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: B7-H2

B7-H2, also called B7RP-1 and ICOSL, is a 60 kDa member of the B7 family of immune costimulatory proteins. It is expressed on resting B cells, dendritic cells, monocytes, alveolar epithelial cells, and placental trophoblasts. B7-H2 interactions with ICOS promote lymphocyte differentiation and activation. Within the ECD, mouse B7-H2 shares 49% and 70% aa sequence identity with human and rat B7-H2, respectively.

  • Long Name:
    B7 Homolog 2
  • Entrez Gene IDs:
    23308 (Human); 50723 (Mouse); 102132485 (Cynomolgus Monkey)
  • Alternate Names:
    B7H2; B7-H2; B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand
Related Research Areas

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