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Mouse Bcl-2 related protein A1 Antibody

Catalog #: MAB851
1 Citation Datasheet / COA / SDS

Discontinued Product

MAB851 has been discontinued.
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Detection of Mouse Bcl‑2 related protein A1 by Western Blot.
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Mouse Bcl-2 related protein A1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Bcl‑2 related protein A1 in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 78616
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant mouse Bcl‑2 related protein A1
Met1-Ser152
Accession # Q07440
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Bcl‑2 related protein A1 antibody by Western Blot. View Larger

Detection of Mouse Bcl‑2 related protein A1 by Western Blot. Western blot shows samples of Recombinant Human Bcl-2 related protein A1 Minus C-terminus, aa 1-152 (Catalog # 1160-A1) (0.3 ng and 1 ng). PVDF membrane was probed with 1 µg/mL Rat Anti-Mouse Bcl-2 related protein A1 Monoclonal Antibody (Catalog # MAB851) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band for Bcl-2 related protein A1 was detected at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl-2 related protein A1

Bcl-2 related protein A1 (abbreviated Bcl2a1 or A1) is an anti-apoptotic outer mitochondrial membrane protein that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. A1 prevents apoptosis by binding to the BH3 region of pro-apoptotic Bcl-2 family members.

Entrez Gene IDs
597 (Human); 12044 (Mouse)
Alternate Names
ACC-1; ACC-2; Bcl-2 related protein A1; BCL2A1; Bcl2L5; Bcl2-L-5; BCL2L5HBPA1; Bcl-2-like protein 5; BCL2-related protein A1; Bfl-1; BFL1bcl-2-related protein A1; GRS; GRSbcl2-L-5; hematopoietic BCL2-related protein A1; Hemopoietic-specific early response protein; Protein BFL-1; Protein GRS

Product Datasheets

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Citation for Mouse Bcl-2 related protein A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Bcl2a1 serves as a switch in death of motor neurons in amyotrophic lateral sclerosis.
    Authors: Crosio C, Casciati A, Iaccarino C, Rotilio G, Carri MT
    Cell Death Differ., 2006-05-19;13(12):2150-3.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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