Mouse C5L2/GPR77 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC4729G-100UG
Detection  of C5L2/GPR77 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Mouse C5L2/GPR77 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
This antibody stains mouse C5L2 transfectants but not irrelevant transfectants.
Source
Monoclonal Rat IgG2B Clone # 468705
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line transfected with mouse C5L2
Met1-Val344
Accession # Q8BW93
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of C5L2/GPR77 in RAW 264.7 Mouse Cell Line by Flow Cytometry. View Larger

Detection of C5L2/GPR77 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse C5L2/GPR77 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC4729G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005).View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: C5L2/GPR77

C5L2, also known as GPR77, is a 45 kDa 7TM receptor that binds the complement proteins C3a, C4a, and C5a as well as the acylation stimulating peptide (ASP). C5L2 is expressed on various hematopoietic cells where it promotes complement-mediated inflammation. ASP binding to C5L2 on adipocytes triggers triglyceride synthesis and glucose uptake and inhibits lipolysis. Human C5L2 shares 60% amino acid sequence identity with mouse and rat C5L2.

Long Name
C5a Anaphylatoxin Chemotactic Receptor C5L2
Entrez Gene IDs
27202 (Human); 319430 (Mouse); 445269 (Rat)
Alternate Names
C5a anaphylatoxin chemotactic receptor C5L2; C5L2; C5L2G-protein coupled receptor 77; G protein-coupled receptor 77; G protein-coupled receptor C5L2; GPF77; GPR77

Product Datasheets

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