Mouse C5L2/GPR77 Alexa Fluor® 488-conjugated Antibody Summary
Accession # Q8BW93
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of C5L2/GPR77 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse C5L2/GPR77 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC4729G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005).View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
C5L2, also known as GPR77, is a 45 kDa 7TM receptor that binds the complement proteins C3a, C4a, and C5a as well as the acylation stimulating peptide (ASP). C5L2 is expressed on various hematopoietic cells where it promotes complement-mediated inflammation. ASP binding to C5L2 on adipocytes triggers triglyceride synthesis and glucose uptake and inhibits lipolysis. Human C5L2 shares 60% amino acid sequence identity with mouse and rat C5L2.
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