Detection of C5L2/GPR77 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse C5L2/GPR77 Monoclonal Antibody (Catalog # MAB4729, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
C5L2, also known as GPR77, is a 45 kDa 7TM receptor that binds the complement proteins C3a, C4a, and C5a as well as the acylation stimulating peptide (ASP). C5L2 is expressed on various hematopoietic cells where it promotes complement-mediated inflammation. ASP binding to C5L2 on adipocytes triggers triglyceride synthesis and glucose uptake and inhibits lipolysis. Human C5L2 shares 60% amino acid sequence identity with mouse and rat C5L2.
C5a Anaphylatoxin Chemotactic Receptor C5L2
Entrez Gene IDs:
27202 (Human); 319430 (Mouse); 445269 (Rat)
C5a anaphylatoxin chemotactic receptor C5L2; C5L2; C5L2G-protein coupled receptor 77; G protein-coupled receptor 77; G protein-coupled receptor C5L2; GPF77; GPR77
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