Mouse Carbohydrate Sulfotransferase 7/CHST7 Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # AF5108AFP488

R&D Systems
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Goat IgG
View all Carbohydrate Sulfotransferase 7/CHST7 Primary Antibodies »

Product Specifications

Specificity

Detects mouse Sulfotransferase 7/CHST7 in direct ELISAs and Western blots. In direct ELISA, less than 1% cross-reactivity with recombinant mouse (rm) CHST1, recombinant human CHST2, rmCHST5, and rmCHST3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Carbohydrate Sulfotransferase 7/CHST7

The CHST family is comprised of 14 genes in both human and mouse. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST7, also designated as chondroitin 6-O-sulfotransferase (C6ST-2), N-acetylglucosamine-6-O-sulfotransferase (GlcNAc6ST-4) and Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST-5), was previously shown to act on chondroitin sulfate, mannose-linked GlcNAc and mucin associated GlcNAc residues (3‑5). The amino acid sequence of mouse CHST7 is 87% identical to that of the human counterpart.

References

  1. Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
  2. Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
  3. Kitagawa, H. et al. (2000) J. Biol. Chem. 275:21075.
  4. Uchimura, K. et al. (2000) Biochem. Biophys. Res. Commun. 274:291.
  5. Bhakta, S. et al. (2000) J. Biol. Chem. 275:40226.
  6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, p.964.
  7. MacRae, I.J. et al. (2000) Biochemistry 39:1613.
  8. Wu, Z.L. et al. (2002) FASEB J. 16:539.

Alternate Names

C6ST2, CHST7, GlcNAc6ST-4, GST-5

Entrez Gene IDs

56548 (Human); 60322 (Mouse); 302302 (Rat)

Gene Symbol

CHST7

UniProt

Q9EP78

Additional Carbohydrate Sulfotransferase 7/CHST7 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Associated Pathways

Articular Cartilage Extracellular Matrix
Articular Cartilage Extracellular Matrix Thumbnail